计算溶液所需的质量、体积或浓度。
BioReagent,for DNA and RNA applications,PCR试剂,分子生物学级,无DNA酶和RNA酶 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
StableLink RT-qPCR Kit (with UDG) 由 Buffer Mix 和 Enzyme Mix 组成,是一款两组分探针法 RT-qPCR 预混液,适用于 RNA 单/多重项目的荧光定量 PCR检测。经优化,本产品的缓冲体系具有同时适合 Taq 酶与逆转录酶扩增的优势,体系的项目普适性强,具有较强的稳定性。
本试剂中引入了 dUTP/热敏 UDG 防污染系统,在室温下即可将含 U 的污染物迅速降解。
适用范围:定性/定量 PCR、多重 PCR。
组分表
FP1509102 | 组分 | 100T | 1000T | 10000T | 存储温度 |
FP1509102A | StableLink RT-PCR Buffer Mix | 1.25 mL | 10×1.25 mL | 125 mL | -20℃ |
FP1509102B | StableLink RT-PCR Enzyme Mix | 100 μL | 1.0 mL | 10 mL | -20℃ |
使用说明
反应体系配制前准备:
1、室温或者4℃溶解反应所需的各种试剂,混匀后备用。
2、参考下表配制 PCR 反应体系:
| 组分 | 加入体积 | 终浓度 |
| StableLink RT-PCR Buffer Mix | 12.5 μL | 1× |
| StableLink RT-PCR Enzyme Mix | 1 μL | 1× |
| 引物探针混合液 | 1 μL | / |
| 模板 | 5 μL | / |
| ddH₂O | To 25 μL | / |
| 终体积 | 25 μL |
注:引物以及模板的用量可自行调整
3、引物、探针和模板的用量可根据项目自行调整,不足的部分可用 ddH₂O 补齐;
4、如需配制其他体积的体系,可基于 25μL 体系用量进行调整,如配制 50μL 体积时,试剂用量相应增加至 2 倍;
5、体系配制完成后,混匀离心后上机检测。
| 步骤 | 温度 | 时间 | 循环 |
| 1 | 50℃ | 10min | 1 |
| 2 | 95℃ | 2min | 1 |
| 3 | 95℃ | 15s | 45 |
| 4 | 60℃ | 40s(收集荧光) | 45 |
注:反应条件根据引物探针和模板情况可自行设置。
The StableLink RT-qPCR Kit (with UDG) is a two-component, probe-based RT-qPCR master mix comprising Buffer Mix and Enzyme Mix. It is designed for sensitive fluorescent quantitative PCR detection in single or multiplex RNA targets. Optimized for performance, the buffer system is uniquely formulated to support both Taq DNA polymerase and reverse transcriptase activity, ensuring broad target compatibility and exceptional reaction stability.
This kit incorporates a dUTP/thermolabile UDG carryover prevention system, enabling rapid degradation of uracil-containing contaminants at room temperature.
Applications: Qualitative/quantitative PCR, multiplex PCR.
Product Component Table
FP1509102 | Components | 100T | 1000T | 10000T | Storage |
FP1509102A | StableLink RT-PCR Buffer Mix | 1.25 mL | 10×1.25 mL | 125 mL | -20℃ |
FP1509102B | StableLink RT-PCR Enzyme Mix | 100 μL | 1.0 mL | 10 mL | -20℃ |
Protocol
Reaction Setup Preparation:
1. Thaw all required reagents at room temperature or 4°C. Mix thoroughly before use.
2. Prepare the PCR Reaction Mixture according to the table below:
| Component | Volume for 25 μL System | Final Concentration |
| StableLink RT-PCR Buffer Mix | 12.5 μL | 1× |
| StableLink RT-PCR Enzyme Mix | 1 μL | 1× |
| Primer/Probe Mix | 1 μL | / |
| Template | 5 μL | / |
| ddH₂O | To 25 μL | / |
| Total Volume | 25 μL |
Note:
"1x" indicates the working concentration of the enzyme mix as provided.
The amounts of primers, probes, and template can be adjusted according to experimental requirements.
Adjust the volumes of primers, probes, and template as needed for your specific assay; use RNase-Free ddH₂O to bring the reaction to the final volume.
To prepare reaction volumes other than 25 μL (e.g., 50 μL), scale the reagent volumes proportionally (e.g., double the volumes for a 50 μL reaction).
3. Mixing and Setup:
After preparing the reaction mixture, mix gently by pipetting or brief vortexing. Centrifuge briefly to collect the contents at the bottom of the tube. Proceed to PCR amplification.
Standard Thermal Cycling Protocol:
| Step | Temperature | Time | Circulation |
| 1 | 50℃ | 10min | 1 |
| 2 | 95℃ | 2min | 1 |
| 3 | 95℃ | 15s | 45 |
| 4 | 60℃ | 40s (Acquire Fluorescence) | 45 |
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | FP1509102 | |
| 分析证书 | FP1509102 |
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