Name: L-谷氨酰胺合成酶 (GS)
SKU: G1374085
Availability: InStock

L-谷氨酰胺合成酶 (GS)

CAS: 9023-70-5 货号: G1374085 EC号: 999-193-0

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,生物活性,重组,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

Glutamine synthetase is an enzyme that converts glutamate and ammonia into glutamine. Glutamine synthetase can inhibit angiogenesis in ocular and inflammatory skin diseases. Glutamine synthetase can also palmitoylate RHOJ, thereby inhibiting endothelial cell migration. Glutamine synthetase can be used in research on various diseases, including cardiovascular and cerebrovascular diseases and cancer.

ATP + L–glutamate + NH₃ → ADP + Orthophosphate + L–glutamine

Properties

Michaelis constant: NH₄Cl 0.05 mM; Glutamic acid 10.9 mM

Isoelectric point: pH 4.85

Molecular weight: 500KDa (TSK G3000SWₓₗ); 53KDa (SDS-PAGE)

Optimum pH: 8–9　　　　　　　　　　　　　　Figure 1

pH stability: 5.0–9.5 (37℃, 60min)　　 Figure 2

Optimum temperature: 60℃ (pH8.0, 50mM Tris–HCl buffer)　 Figure 3

Thermal stability: Stable at 60℃ and below (pH8.0, 10min)　 Figure 4

Storage stability : −20℃

Effect of metal ions: See Table 1

Applications

This enzyme is useful for elimination of ammonia in a diagnostic reagent.

Table 1. Effect of metal ions GS activity

Metal	%	Metal	%
None	100	AgCl	82
NaCl	101	CsCl	90
KCl	97	NH₄Cl	69
EDTA	0	ZnSO₄	0
LiCl	92	CuSO₄	0

※ 10mM Metal ion with 5mM MgCl₂

Assay

■ Principle

The assay is based on the decrease in absorbance at 340 nm as the consumption of NADH proceeds in the following reactions:

PK :Pyruvate kinase

LDH:Lactate dehydrogenase

PEP:Phosphoenol pyruvate

■ Unit definition

One unit is defined as the amount of enzyme which produces 1 μmole of glutamine per minute at 37 ℃ under the conditions specified in the assay procedure.

■ Reagents

1. Reaction mixture

0.2 M Tris-HCl buffer pH 8.0	0.75 ml
60 mM ATP solution pH 8.0	0.30 ml
1 M NH₄Cl solution	0.30 ml
30 mM PEP solution	0.20 ml
7.86 mM NADH solution	0.10 ml
0.6 M MgCl₂ solution	0.15 ml
100 U/ml PK¹⁾ solution	0.10 ml
340 U/ml LDH²⁾ solution	0.10 ml
0.45 M Glutamic acid Na	0.60 ml
Distilled water	0.40 ml

1): 100U/ml PK Stock solution (2000U/ml) of PK with distilled water to make 20-fold solution.
2): 340U/ml LDH Stock solution (5500U/ml) of LDH with distilled water to make 16-fold solution.

2. Enzyme dilution buffer

10mM Tris–HCl buffer pH8.0

■ Enzyme solution

Accurately weigh about 10 mg of the sample and add enzyme dilution buffer to make a total of 10 ml. Dilute it with enzyme dilution buffer to adjust the concentration as required.

■ Procedure

1. Pipette accurately 3.0 ml of reaction mixture into a small test tube and Preincubate at 37℃

2. After 5min. add accurately 60 μl of enzyme solution and mix to start the reaction at 37℃ ※ In the case of a test blank add 60 μl of enzyme dilution buffer in place of enzyme solution

3. After starting the reaction, measure the rate of increase per minutes in absorbance at 340 nm. The rate must be measured within the linear portion of the absorbance curve. (Ex. Linear range from 3 min to 6 min)

Absorbance

sample : As/min

blank : Ab/min

△A/min = (As/min − Ab/min) ≤ 0.15 Abs/min

■ Calculation

Activity (U/mg) = (ΔA/min/ 6.3) × (3.06 / 0.06) × (1 / X)

6.3: millimolar extinction coefficient of NADH at 340nm (cm² / μmol)

3.06: final volume (ml)

0.06: volume of emzyme solution (ml)

X: concentration of the sample in enzyme solution

规格

产品名称

L-谷氨酰胺合成酶 (GS)

别名

谷氨酰胺合成酶 | 谷氨酸氨连接酶

英文别名

L-Glutamate: ammonia Ligase (ADP-forming) | L-Glutamate:ammonia ligase (ADP-forming) | glutamine synthetase | glutamate--ammonia ligase | glnA

规格或纯度

生物活性, 重组, ActiBioPure™, 高性能, EnzymoPure™, ≥15 U/mg enzyme powder

生物活性

≥15 U/mg enzyme powder

种属

芽孢杆菌属(Bacillus sp.)

来源

重组表达

SDS-PAGE

53 kDa

CAS编号和信息

9023-70-5

酶学委员会编号

EC 6.3.1.2

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥15 U/mg enzyme powder

复溶

Reconstitute in 10mM Tris–HCl buffer pH8.0 to a concentration of 1 mg/ml.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃；复溶后建议分装，避免反复冻融。

单位定义

One unit is defined as the amount of enzyme which produces 1 μmole of glutamine per minute at 37 °C under the conditions specified in the assay procedure.

技术文档

📋 安全数据表 (SDS)

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✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

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📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

g1374085 lglutamine synthetase manual.pdf

g1374085 l谷氨酰胺合成酶说明书.pdf

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L-谷氨酰胺合成酶 (GS)

储存与运输

质量文档

文献证明

概述

Assay

■ Principle

■ Unit definition

■ Reagents

■ Enzyme solution

■ Procedure

■ Calculation

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

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