计算溶液所需的质量、体积或浓度。
N-乙酰葡萄糖胺激酶 (EcNagK) ,EC 2.7.1.59,来源于E. coli,可以用于 N 乙酰葡萄糖的磷酸化。本产品为大肠杆菌重组表达。
反应式
N‑acetylglucosamine kinase (EcNagK), EC 2.7.1.59, derived from E. coli, which catalyzes the phosphorylation of N‑acetylglucosamine. This product is recombinantly expressed in E. coli.
Reaction Equation
GlcNAc + ATP → GlcNAc‑6‑P + ADP
Usage
Add substrates and 5 mM MgCl₂ to the reaction system. Appropriately add buffer salts to adjust pH to 7.5, then add an appropriate amount of EcNagK according to reaction requirements. After mixing, incubate the sample at 37 °C for 2‑4 h.
Notes
1. For scaled‑up reactions, incubation time or enzyme dosage shall be adjusted according to practical conditions; low‑speed shaking mixing is recommended.
2. Most common biological buffers such as Tris or PBS are applicable. Buffers outside this pH range (e.g., acetate buffer) may also work, whereas incubation time and enzyme dosage need to be optimized empirically.
Precautions
For your safety and health, wear a lab coat and disposable gloves during operation.
This product is for research use only!
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | G1433378 |
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