计算溶液所需的质量、体积或浓度。
EnzymoPure™,生物活性,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
N-乙酰葡萄糖胺激酶 (EcNagK) ,EC 2.7.1.59,来源于E. coli,可以用于 N 乙酰葡萄糖的磷酸化。本产品为大肠杆菌重组表达。
反应式
N‑acetylglucosamine kinase (EcNagK), EC 2.7.1.59, derived from E. coli, which catalyzes the phosphorylation of N‑acetylglucosamine. This product is recombinantly expressed in E. coli.
Reaction Equation
GlcNAc + ATP → GlcNAc‑6‑P + ADP
Usage
Add substrates and 5 mM MgCl₂ to the reaction system. Appropriately add buffer salts to adjust pH to 7.5, then add an appropriate amount of EcNagK according to reaction requirements. After mixing, incubate the sample at 37 °C for 2‑4 h.
Notes
1. For scaled‑up reactions, incubation time or enzyme dosage shall be adjusted according to practical conditions; low‑speed shaking mixing is recommended.
2. Most common biological buffers such as Tris or PBS are applicable. Buffers outside this pH range (e.g., acetate buffer) may also work, whereas incubation time and enzyme dosage need to be optimized empirically.
Precautions
For your safety and health, wear a lab coat and disposable gloves during operation.
This product is for research use only!
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Bioactive 级别指南 → 查看 Recombinant 级别指南 → 查看 EnzymoPure™ 级别指南 →