计算溶液所需的质量、体积或浓度。
EnzymoPure™,生物活性,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
半乳糖激酶 (BiGalK), EC 2.7.1.6,来源于Bifidobacterium infantis,催化D-半乳糖(Gal)磷酸化为D-Gal-1-P。本产品为大肠杆菌重组表达,纯度高,活性好。
反应式
Gal + ATP → Gal-1-P + ADP
使用方法
按照反应体系加入底物、5mM MgCl₂,可适当加入缓冲盐如:Tris-HCL,调节 pH 为 7.5,加入适量 AtGalAK(根据反应需求);
混匀后,将样品置于 37℃孵育 2-4h。
终止反应;沸水浴 5min,10000rpm 离心 10min。取上清 HPLC/TLC 分析。
注
体系放大时候,孵育时间或酶量需要根据实际情况调整,可低速震荡混匀。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途!
Galactokinase (BiGalK), EC 2.7.1.6, derived from Bifidobacterium infantis. It catalyzes the phosphorylation of D‑galactose (Gal) into D‑Gal‑1‑P. This product is recombinantly expressed in E. coli with high purity and excellent activity.
Reaction Equation
Gal + ATP → Gal‑1‑P + ADP
Usage
Add substrates and 5 mM MgCl₂ to the reaction system. Add appropriate buffer salt such as Tris‑HCl to adjust pH to 7.5, then add a suitable amount of BiGalK according to reaction requirements. After mixing, incubate the sample at 37 °C for 2‑4 h.
Terminate the reaction in a boiling water bath for 5 min, centrifuge at 10000 rpm for 10 min. Collect the supernatant for HPLC/TLC analysis.
Note
For scaled‑up reactions, incubation time or enzyme dosage should be adjusted according to practical conditions; low‑speed shaking mixing is recommended.
Precautions
For your safety and health, wear a lab coat and disposable gloves during operation.
This product is for research use only!
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Bioactive 级别指南 → 查看 Recombinant 级别指南 → 查看 EnzymoPure™ 级别指南 →