α-Glucosidase-IN-36 (compound 5g) is a potent α-glucosidase inhibitor, with an IC 50 of 6.69 ± 0.18 μM, K i and K is of 1.65 μM and 4.54 μM, respectively. α-Glucosidase-IN-36 may inhibit α-glucosidase activity by binding with its active site as well as changing the secondary structure of α-glucosidase. α-Glucosidase-IN-36 can be used for type 2 diabetes mellitus (T2DM) research.