鲁哥氏染色液也称Lugol's碘液或鲁哥氏溶液或鲁哥试剂，是碘和碘化钾的水溶液，是Lugol在1829年发明，并以其姓名命名。Lugol's碘液过去经常被用作消毒剂和杀菌剂，用于饮用水的应急消毒，实验室中用于检测淀粉等多种物质。
阿拉丁鲁哥氏染色液 (5%) 采用经典配方，其用途如下：检测叶片、食物等样品中的淀粉；细胞染色，使细胞核着色从而更易观察；Schiller Test，在阴道镜中检查阴道和子宫颈是否癌变。正常阴道组织由于其高糖原质含量而被染成棕褐色，癌变的细胞则不会被染上颜色，与周围组织相比显现浅色；氧化性杀菌剂等。 鲁哥氏染色液 (5%) 适合于作为媒染剂与结晶紫、沙黄等联合使用，对革兰氏细菌进行染色，其碘的含量为5%。 鲁哥氏染色液，特别适合作为媒染剂对子宫颈进行涂抹染色，进而判断宫颈癌的可能。本试剂仅用于科研用途，不可用于临床诊断或其他用途。
操作步骤(仅供参考)：
1、根据实验具体要求操作或参考如下步骤。
2、使用盐水棉球或干棉签轻轻插拭子宫颈，注意不应太用力，避免子宫颈上皮破损。
3、使用鲁哥氏染色液 (5%) 浸泡的棉球涂抹子宫颈，棉球应足够大，全部覆盖子宫颈。轻轻用棉球上下左右按压子宫颈 (包括子宫颈外口)，使鲁哥氏染色液充分渗透子宫颈上皮。一分钟后，用普通光源照明，直接观察子宫颈上皮对鲁哥氏染色液的反应。
4、根据未着色的程度、表面形态、边界状况、大小、与鳞柱交界的距离等，做出初步诊断。正常子宫颈上皮在涂碘后为赤褐色或黑色，未着色区或仅部分碘染为可疑病变区。CIN病变或浸润癌区域呈现厚重的芥末黄或桔黄色白斑。在阴道镜检查中应用碘溶液有助于识别醋酸方法遗漏的病变区域，并将更清晰地显示异常范围。该检测结果分为VILI阴性、VILI阳性、可疑癌。结果应记入临床检查表。
染色结果：
注意事项：
1、本试剂仅用于科研用途，不可用于临床诊断。
2、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Lugol's Staining Solution, also known as Lugol's Iodine, Lugol's Solution or Lugol's Reagent, is an aqueous solution of iodine and potassium iodide. It was invented by Lugol in 1829 and named after him. In the past, Lugol's Iodine was often used as a disinfectant and bactericide for emergency disinfection of drinking water, and in laboratories for detecting starch and various other substances.
Aladdin Lugol's Staining Solution (5%) adopts a classic formula and has the following applications: detecting starch in samples such as leaves and food; cell staining to stain cell nuclei for easier observation; the Schiller Test, which is used in colposcopy to examine the vagina and cervix for cancerous changes. Normal vaginal tissues are stained brown due to their high glycogen content, while cancerous cells are not stained and appear pale in contrast to the surrounding tissues; acting as an oxidizing bactericide, etc. Lugol's Staining Solution (5%) is suitable for use as a mordant in combination with crystal violet, safranine and other dyes for staining Gram bacteria, with an iodine content of 5%. Lugol's Staining Solution is particularly suitable for use as a mordant for smearing and staining the cervix to determine the possibility of cervical cancer. This reagent is for research use only and is not intended for clinical diagnosis or any other purposes.
Operating Procedures (For Reference Only):
1. Perform the operation according to the specific experimental requirements or refer to the steps below.
2. Gently swab the cervix with a normal saline-moistened cotton ball or dry cotton swab. Do not apply excessive force to avoid damaging the cervical epithelium.
3. Smear the cervix with a cotton ball soaked in Lugol's Staining Solution (5%). Ensure the cotton ball is large enough to fully cover the cervix. Gently press the cervix (including the external cervical os) with the cotton ball in upward, downward, leftward and rightward directions to allow the Lugol's Staining Solution to fully penetrate the cervical epithelium. After one minute, illuminate the area with a regular light source and directly observe the reaction of the cervical epithelium to the Lugol's Staining Solution.
4. Make a preliminary diagnosis based on the degree of non-staining, surface morphology, border condition, size, distance from the squamocolumnar junction, and other factors. Normal cervical epithelium turns reddish-brown or black after iodine application. Non-stained areas or partially iodine-stained areas are considered suspicious lesion regions. Areas with cervical intraepithelial neoplasia (CIN) or invasive carcinoma present as thick mustard-yellow or orange-yellow leukoplakia. The application of iodine solution during colposcopy helps identify lesion areas missed by the acetic acid method and can more clearly delineate the scope of abnormalities. The test results are classified as VILI negative, VILI positive, or suspected cancer, and should be recorded in the clinical examination form.
Staining Results:
Cartilage matrix, cytoplasm
| Red
|
Precautions:
1. This reagent is intended for research use only and not for clinical diagnosis.
2. For your safety and health, wear a lab coat and disposable gloves during operation.
ZJ26F1039985
危险品化学品经营许可证（带存储）