计算溶液所需的质量、体积或浓度。
GUS染色试剂盒（即用型）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
GUS染色试剂盒（即用型）采用经典的组织化学方法，通过与底物的颜色反应来对植物中gus基因融合标记进行快速检测。
gus基因存在于大肠杆菌E.coli等一些细菌基因组内，其表达产物β-葡萄糖苷酸酶（GUS，β-glucuronidase，β-D-葡萄糖苷酸酶）是一种水解酶，能催化许多β-葡萄糖苷酯类物质的水解。因为绝大多数植物细胞内不存在β-葡萄糖苷酸酶的背景活性，因此gus基因是转基因植物最常用的报告基因之一，尤其是在研究外源基因瞬时表达的转化实验中被广泛应用。β-葡萄糖苷酸酶可以将5-溴-4-氯-3-吲哚-β-葡萄糖苷酸酯（X-Gluc）分解为蓝色的不溶物质，因此用含有X-Gluc底物的染色液浸泡转gus基因植物，具有GUS活性的部位或位点呈现蓝色或蓝色斑点，可用肉眼或显微镜观察到。其检测方法简单、快速、灵敏、稳定， 且背景活性低。
本试剂盒包含GUS染色的全部试剂，使用方便，只需将配制好的X-Gluc Solution和GUS Buffer按照比例混合即配成GUS染色液。
| G1508328 | Component | 50 mL | 100 mL | Storage |
| G1508328A | X-Gluc | 1 EA | 2 EA | -20℃. Store in the dark. |
| G1508328B | X-Gluc Solution | 1 mL | 1 mL×2 | RT. Store in the dark. |
| G1508328C | GUS Buffer | 50 mL | 100 mL | 2-8℃. Store in the dark. |
自备仪器与试剂
- 70%乙醇、去离子水
- 小瓶或多孔板、显微镜
使用说明（仅供参考）
1、配制X-Gluc溶液 (50×)：取1ml X-Gluc Solution完全加至1支X-Gluc中，充分溶解，即获得X-Gluc溶液 (50×)。配制好的X-Gluc溶液 (50×) -20℃保存，1个月有效。
2、配制X-Gluc染色液：取适量的X-Gluc溶液 (50×) 和GUS Buffer，按1：49比例充分混匀，配制成X-Gluc染色液，如取0.2ml X-Gluc溶液 (50×) 加入到9.8ml GUS Buffer中，即配成10ml GUS染色液，该染色液最好现用现配，短期贮存可以4℃保存3天。
3、取适量待染叶片等组织加入适量GUS染色液使其完全浸没组织。
4、37℃孵育1~24h，随着孵育时间的延长，蓝色渐渐出现，当表达量较高时GUS活性的部位或位点呈现蓝色或蓝色斑点。
5、用70%乙醇脱去样本的叶绿素，一般样本浸没于乙醇1～3h，至阴性对照呈白色。如有必要可重复该脱色步骤，以便彻底清除叶绿素；样本保存于乙醇中，可用肉眼或普通光学显微镜下观察，白色背景上的蓝色即为GUS表达位点。
注意事项
1、GUS染色液最好冰上配制，现配现用。配制后用不完可-20℃避光保存，如果溶液由无色变为红色或紫红色应弃用。
2、由于组织特异性等原因，蓝色颜色反应可能不完全一致，应注意摸索具体实验条件。
3、拟南芥的根、花和叶片以及烟草幼苗根就可直接染色；烟草和马铃薯这些植物的茎和叶须在染色前切成薄片（1~3mm）。
4、当操作大的组织和样品时可以选用真空渗入法来帮助底物和酶渗入细胞。
5、GUS染色液（即用型）染色灵敏度较好，但有时会出现非特异性反应（即假阳性）。
6、试剂开封后请尽快使用，以防影响后续实验效果。
GUS Staining Kit (Ready-to-Use) employs a classic histochemical method to rapidly detect gus gene fusion markers in plants through a color reaction with a substrate.
The gus gene is present in the genomes of certain bacteria such as Escherichia coli. Its product, β-glucuronidase (GUS), is a hydrolytic enzyme that catalyzes the hydrolysis of many β-glucuronide esters. Because most plant cells lack endogenous β-glucuronidase background activity, the gus gene is one of the most commonly used reporter genes in transgenic plants, especially widely applied in studies of transient expression of foreign genes. β-Glucuronidase can cleave 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-Gluc) into an insoluble blue product. Therefore, when transgenic plants carrying the gus gene are soaked in a staining solution containing X-Gluc substrate, tissues or sites with GUS activity appear blue or show blue spots, which can be observed with the naked eye or under a microscope. The detection method is simple, fast, sensitive, stable, and has low background activity.
This kit contains all reagents required for GUS staining. It is convenient to use: simply mix the prepared X-Gluc Solution and GUS Buffer in the specified ratio to prepare the GUS staining solution.
| G1508328 | Component | 50 mL | 100 mL | Storage |
| G1508328A | X-Gluc | 1 EA | 2 EA | -20℃. Store in the dark. |
| G1508328B | X-Gluc Solution | 1 mL | 1 mL×2 | RT. Store in the dark. |
| G1508328C | GUS Buffer | 50 mL | 100 mL | 2-8℃. Store in the dark. |
Reagents, consumables and Equipments not provided
70% ethanol, Deionized water
Small vials or multi-well plates, Microscope
Instructions for Use (For Reference Only)
Prepare X-Gluc Solution (50×): Add 1 mL of X-Gluc Solution completely to one vial of X-Gluc. Dissolve thoroughly to obtain the X-Gluc Solution (50×). Store the prepared X-Gluc Solution (50×) at -20°C; it is stable for 1 month.
Prepare X-Gluc Staining Solution: Take an appropriate amount of X-Gluc Solution (50×) and GUS Buffer, mix thoroughly at a ratio of 1:49 to prepare the X-Gluc staining solution. For example, add 0.2 mL of X-Gluc Solution (50×) to 9.8 mL of GUS Buffer to prepare 10 mL of GUS staining solution. It is best to prepare the staining solution fresh for each use. For short-term storage, it can be kept at 4°C for up to 3 days.
Staining: Add an appropriate amount of tissue (e.g., leaves) to be stained into sufficient GUS staining solution to completely submerge the tissue.
Incubation: Incubate at 37°C for 1–24 hours. Blue color gradually appears with extended incubation time. Sites with high GUS activity will show blue or blue spots.
Decolorization: Remove chlorophyll from the sample using 70% ethanol. Generally, submerge the sample in ethanol for 1–3 hours until negative controls turn white. If necessary, repeat the decolorization step to thoroughly remove chlorophyll. Store the sample in ethanol. Observe with the naked eye or under a standard light microscope; blue color on a white background indicates GUS expression sites.
Notes
It is best to prepare the GUS staining solution on ice and use it immediately. Unused prepared solution can be stored at -20°C protected from light. Discard if the solution turns red or purplish-red.
Due to tissue specificity and other factors, the intensity of the blue color reaction may vary. Optimal experimental conditions should be determined empirically.
Tissues such as Arabidopsis roots, flowers, leaves, and tobacco seedling roots can be stained directly. Stems and leaves of plants like tobacco and potato should be cut into thin slices (1–3 mm) before staining.
For large tissues or samples, vacuum infiltration can be used to facilitate substrate and enzyme penetration into cells.
The GUS Staining Kit (Ready-to-Use) offers good staining sensitivity, but non-specific reactions (false positives) may occasionally occur.
Use reagents as soon as possible after opening to avoid affecting subsequent experimental results.
规格
别名
GUS染色试剂盒 | 转基因植物报告基因试剂盒 | GUS染色液
规格或纯度
BioReagent,用于显微镜,生物染色剂
英文名称
GUS Staining Kit (Ready-to-use)
应用
细胞染色
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光,室温,-20°C储存
运输条件
超低温运输
稳定性与储存
各组分在相应的储存条件下保质期1年。
技术文档
高级数据
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 G2610351 |
分析证书 | G1508328 |
| E2625663 |
分析证书 | G1508328 |
| ZJ26F0332890 |
分析证书 | G1508328 |
| ZJ26F0332889 |
分析证书 | G1508328 |
| ZJ26F0332888 |
分析证书 | G1508328 |
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