计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
BioReagent,生物染色剂 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 291 篇同行评审文献引用。
概述
孔雀石绿又称孔雀绿，是人工合成的染料，CAS号为569-64-2，分子量为364.92。甘油-孔雀绿染色液主要由甘油、孔雀绿、去离子水组成，可用于检查粪便的各种蠕虫卵。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备仪器和试剂
- 不锈钢、塑料或纸平板
- 亲水性玻璃纸条
操作步骤（仅供参考）
1、准备玻璃纸条：将玻璃纸浸润于甘油孔雀绿染色液至少大于24h。
2、置少量粪便样本于报纸或小纸片上，用滤网在粪便标本上加压，使部分粪便样本通过滤网积聚于网上。
3、用刮片横刮滤网以收集筛过的粪便样本。
4、取带孔平板置于载玻片中央位置，用刮片时孔内填满粪便样本，并用刮片边缘横刮板面以去除孔边过多的粪便。
5、小心取下平板，使粪便样本呈矮小圆柱状留在玻片上。
6、玻璃纸条浸泡于甘油孔雀绿染色液，取出并覆盖于粪便上。
7、翻转玻片，在另一张玻片或在表面平滑、坚硬的物体上，朝向玻璃纸条挤压粪便样本，以使标本在玻片与玻璃纸条间均匀散开。澄清后，应能透过涂片读出本说明书上的字迹。
8、轻轻从侧面滑动并移下上层玻片，避免与玻璃纸条分离或使之掀起，将玻片置于实验台上，玻璃纸条面朝上。目的玻片（除钩虫卵外）应置于室温一至数小时，以使样本清晰。
9、以上下或横向移动方式检查涂片，并报告所发现的每种虫卵的计数，然后乘以适用的数值得出每克粪便中虫卵的数目。如使用20mg平板，乘以50；使用41.7mg平板，乘以24；使用50mg平板，乘以20。
注意事项
1、刮片和滤网经仔细清洗后，可重复使用。
2、粪便样本较干燥时，覆盖粪便的玻璃纸条必须湿润；如为软便，覆盖粪便的玻璃纸条的水分可略少。在干燥的气候条件下，过多的甘油只能延缓而不能防止粪便样本的干燥。
3、本法制片中的蛔虫及鞭虫卵可保存较长时间：血吸虫卵可保存数月：钩虫卵在制片30~60min后往往就观察不到了。
4、该染色法对粪膜厚度和透明时间有要求，如粪膜厚透明时间短，虫卵难以发现；如透明时间长则虫卵变型，不易辨认。如果检查钩虫卵，透明时间宜控制在30min以内。
5、为了您的安全与健康，请穿实验服并佩戴一次性手套操作。
6、本产品仅用于科研，严禁他用。
Malachite Green, also known as Aniline Green, is a synthetic dye with CAS No. 569-64-2 and a molecular weight of 364.92. Glycerin-Malachite Green Staining Solution is mainly composed of glycerin, malachite green, and deionized water, and can be used to examine various helminth eggs in feces. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Reagents, consumables and Equipments not provided
Stainless steel, plastic, or paper plate
Hydrophilic glass paper strips
Operating Steps (For Reference Only)
Prepare glass paper strips: Soak the glass paper in Glycerin-Malachite Green Staining Solution for at least 24 hours.
Place a small amount of fecal sample on newspaper or a small paper sheet. Press a filter screen onto the fecal specimen so that part of the sample passes through and accumulates on the screen.
Use a spatula to scrape across the filter screen to collect the sieved fecal sample.
Place a perforated plate in the center of a glass slide. Fill the hole with the fecal sample using the spatula, and scrape the plate surface with the edge of the spatula to remove excess sample around the hole.
Carefully remove the plate, leaving a small cylindrical fecal sample on the slide.
Take a glass paper strip soaked in the staining solution and cover the fecal sample with it.
Flip the slide and press the fecal sample against another slide or a smooth, hard surface to spread the specimen evenly between the slide and the glass paper strip. After clarification, the text on this instruction sheet should be readable through the smear.
Gently slide and remove the top slide from the side, avoiding separation from or lifting of the glass paper strip. Place the slide on the bench with the glass paper strip facing upward. For most eggs (except hookworm eggs), allow the slide to stand at room temperature for one to several hours until the sample clears.
Examine the smear by moving up and down or side to side. Report the count of each type of egg found, then multiply by the appropriate factor to obtain the number of eggs per gram of feces:
For a 20 mg plate, multiply by 50.
For a 41.7 mg plate, multiply by 24.
For a 50 mg plate, multiply by 20.
Precautions
The spatula and filter screen can be reused after careful cleaning.
For dry fecal samples, the covering glass paper strip must be well moistened. For soft stools, the glass paper strip can be slightly less moist. In dry climates, excess glycerin can only delay, not prevent, drying of the fecal sample.
In preparations made by this method:
Ascaris and Trichuris eggs can be preserved for a long time.
Schistosome eggs can be preserved for several months.
Hookworm eggs often become unobservable 30–60 minutes after preparation.
This staining method requires specific fecal film thickness and clarification time. If the film is too thick and clarification time is too short, eggs may be difficult to detect. If clarification time is too long, eggs may deform and become hard to recognize. For examining hookworm eggs, the clarification time should be controlled within 30 minutes.
For your safety and health, please wear a lab coat and disposable gloves during operation.
This product is for research use only; any other use is strictly prohibited.
规格
别名
甘油-孔雀石绿染色液
英文别名
Glycerol-Malachite Green Stain
规格或纯度
BioReagent,生物染色剂
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Glycerol-Malachite Green Staining Solution
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
EC号
209-322-8
分子类型
生物试剂/缓冲液
Isomeric SMILES
CN(C)C1=CC=C(C=C1)C(=C2C=CC(=[N+](C)C)C=C2)C3=CC=CC=C3.[Cl-]
UN Number
Packing Group
I
分子量
364.92
Reaxy-Rn
3580148
Reaxys-RN link address
https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=3580148&ln=
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
熔点
158-160° C
安全和危险性（GHS）
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F1039698 |
分析证书 | G1510334 |
| ZJ26F0332384 |
分析证书 | G1510334 |
技术文档和文章
此产品的引用文献
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 569-64-2。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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