洋红是一种比较常用的碱性染料,常用于细胞核染色、染色体的固定和染色,在观察植物细胞有丝分裂时对染色体进行染色,需要用碱性染液,此时可以使用醋酸洋红或龙胆紫染液;花粉检测中可以采用醋酸洋红检测花粉是否处于单核期。
阿拉丁格氏铵矾洋红染色液主要由高浓度硫酸铝铵、洋红(也称胭脂红)等组成,pH呈酸性,格氏铵矾洋红染色液主要用于花粉、动植物组织切片等样本中细胞学的染色,不易发生过染。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
载玻片、盖玻片、光学显微镜
操作步骤(仅供参考):
1、花粉类颗粒:取少量花粉物质置于载玻片,滴加适量铁矾醋酸洋红染色液,充分混合,立即盖片染色15~60min后,吸去多余液体,显微镜下观察。
2、动植物切片:常规处理 (如脱蜡至水),滴格氏铵矾洋红染色液染色10~30min,自来水冲洗,封片,显微镜下观察。
3、部分新鲜植物组织 (如洋葱表皮),直接浸染于格氏铵矾洋红染色液10~30min,自来水冲洗2~3min,置于载玻片并盖上盖玻片,显微镜观察。
4、如果效果不佳,在酒精灯上迅速来回轻烤几次,以破坏染色质,使细胞核着色。
注意事项:
1、染完色后应立即显微镜下观察。
2、由于该试剂含有高浓度弱酸,有刺激性气味,请注意自我保护。
3、注意密闭保存,否则染色效率会下降。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Carmine is a commonly used basic dye, which is often applied for nuclear staining, as well as chromosome fixation and staining. When observing plant cell mitosis, alkaline staining solutions are required for chromosome staining, and acetocarmine or gentian violet staining solution can be used at this time. In pollen detection, acetocarmine can be adopted to determine whether pollen is in the uninucleate stage.
Aladdin Grignard Ammonium Alum Magenta Stain Solution is mainly composed of high-concentration ammonium aluminum sulfate and carmine (also known as cochineal). It is acidic in pH, and is primarily used for cytological staining of samples such as pollen, animal and plant tissue sections, with low risk of over-staining. This reagent is for research use only and not suitable for clinical diagnosis or other purposes.
Materials to Be Prepared by the User:
Glass slides, coverslips, optical microscope
Operating Procedures (For Reference Only):
1. Pollen grains: Place a small amount of pollen on a glass slide, add an appropriate volume of ferric alum acetocarmine staining solution, mix thoroughly, cover with a coverslip immediately and stain for 15–60 minutes. Then absorb the excess liquid and observe under a microscope.
2. Animal and plant tissue sections: Perform routine treatment (e.g., deparaffinize to water), add Gregory’s alum carmine staining solution dropwise and stain for 10–30 minutes. Rinse with tap water, mount the slide, and observe under a microscope.
3. Some fresh plant tissues (e.g., onion epidermis): Immerse the tissue directly in Gregory’s alum carmine staining solution for 10–30 minutes, rinse with tap water for 2–3 minutes, place on a glass slide, cover with a coverslip, and observe under a microscope.
4. If the staining effect is unsatisfactory: Gently and quickly pass the slide back and forth over an alcohol lamp several times to disrupt the chromatin and enhance nuclear staining.
Precautions:
1. Observe the sample under a microscope immediately after staining.
2. Since this reagent contains high-concentration weak acid and has an irritating odor, please take appropriate personal protective measures.
3. Store the reagent in a sealed container; otherwise, the staining efficiency will decrease.
4. For your safety and health, wear a lab coat and disposable gloves during operation.