BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
γ-谷氨酰转肽酶（γ-GT，EC 2.3.2.2）是一种广泛存在于生物体内的膜结合糖蛋白，属于转移酶家族。它是γ-谷氨酰循环中的关键酶，催化谷胱甘肽（GSH）降解。γ-GT 催化 GSH 或者其他γ-谷氨酰基化合物上的γ-谷氨酰基转移到受体，也可以催化 GSH 和其他γ-谷氨酰基化合物的水解，产生谷氨酸盐，在细胞外GSH新陈代谢中起了重要的作用。 本试剂盒检测原理是γ-GT 催化谷氨酰对硝基苯胺中的γ-谷氨酰基转移给N-甘氨酰甘氨酸，生成黄色产物对硝基苯胺，在 405 nm 有特征吸收光；通过测定 405 nm 光吸收增加速率，来计算γ-GT 酶活性。
γ-Glutamyl transpeptidase (γ-GT, EC 2.3.2.2) is a membrane-bound glycoprotein widely present in living organisms and belongs to the transferase family. It is a key enzyme in the gamma-glutamyl cycle, catalyzing the degradation of glutathione (GSH). γ-GT catalyzes the transfer of the γ-glutamyl group from GSH or other γ-glutamyl compounds to an acceptor, and can also catalyze the hydrolysis of GSH and other γ-glutamyl compounds, producing glutamate. It plays an important role in extracellular GSH metabolism. The detection principle of this kit is as follows: γ-GT catalyzes the transfer of the γ-glutamyl group from γ-glutamyl p-nitroanilide to N-glycylglycine, generating the yellow product p-nitroaniline, which exhibits characteristic absorption at 405 nm. The enzymatic activity of γ-GT is calculated by measuring the rate of increase in absorbance at 405 nm.
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