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β-半乳糖苷酶（β-GAL, EC 3.2.1.23）是一种水解酶，广泛存在于动物、植物、微生物和培养细胞中，能够催化β-半乳糖苷化合物中β-半乳糖苷键水解，此外还具有转半乳糖苷的作用。β-GAL 不仅可以为植物的快速生长释放储存的能量，还能在正常的多糖代谢、细胞壁组分代谢以及衰老时细胞壁降解过程中多糖、糖蛋白以及半乳糖脂末端半乳糖残基的水解，释放自由的半乳糖。β-GAL 能够分解对-硝基苯-β-D-吡喃半乳糖苷生成对-硝基苯酚，后者在 400 nm 处有最大吸收峰，通过测定吸光值升高速率来计算β-GAL 的活性。
β-Galactosidase (β-GAL, EC 3.2.1.23) is a hydrolytic enzyme widely present in animals, plants, microorganisms, and cultured cells. It catalyzes the hydrolysis of β-galactosidic bonds in β-galactoside compounds and additionally possesses transgalactosylation activity. β-GAL not only releases stored energy for rapid plant growth but is also involved in normal polysaccharide metabolism, cell wall component turnover, and the hydrolysis of terminal galactose residues in polysaccharides, glycoproteins, and galactolipids during cell wall degradation in senescence, thereby releasing free galactose. β-GAL catalyzes the hydrolysis of p-nitrophenyl-β-D-galactopyranoside to generate p-nitrophenol, which exhibits a maximum absorption peak at 400 nm. The activity of β-GAL is calculated by measuring the rate of increase in absorbance at this wavelength.
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