计算溶液所需的质量、体积或浓度。
Glu广泛存在于动物、植物、微生物和培养细胞中,不仅是组成蛋白质的20种氨基酸之一,而且通过转氨基作用参与多种氨基酸合成,是生物体内主要氨基来源之一。此外,Glu还是味精的主要有效成分,常用做食品添加剂以及香料生产。 测定原理:利用专用提取液提取,然后用显色剂进行显色,显色后在570nm下进行测定。
Glutamate (Glu) is widely present in animals, plants, microorganisms, and cultured cells. It is not only one of the 20 amino acids that constitute proteins but also participates in the synthesis of various amino acids through transamination, serving as a primary source of amino groups in living organisms. Additionally, Glu is the main active component of monosodium glutamate, commonly used as a food additive and in flavor production. Assay Principle: Samples are extracted using a specialized extraction buffer, followed by color development with a chromogenic agent. Absorbance is measured at 570 nm after color development.