计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
产品优势
a) 标记工艺简单
金微子纳米微球与传统乳胶微球相比，标记工艺更简单，离心更容易，离心力不超过 500Xg，不需要额外昂贵的离心设备。
b) 色彩更加多样
金微子颜色多样，可满足客户个性化定制，保留了胶体金易于标记，离心不脱色显色稳定的优点。
c) 灵敏度更高
与同粒径的乳胶微球项目，灵敏度更优，且显著优于常规胶体金。
d) 均一性好
单分散性好，PDI＜0.2。
e) 适配性强
在低爬速的膜上跑板依然优秀。
推荐标记方法
以抗体标记为例：
i. 稀释：取 100uL 原液，加水 280uL 水至终体积 380uL。
ii. 标 记 ：将待标记的抗体（ 或抗 原 ）按 终浓 度10ug/mL~50ug/mL 的量加入到稀释后的金微子中，震荡混匀。
iii. pH 调节：在上述加入抗体后的金微子中，建议加入 20uL 的缓冲液调整 pH。（浓度 0.2 mol/L，pH范围 4~10），室温震荡反应 30 分钟。
iv. 封闭：加入终浓度 5‰的 BSA，以 400uL 稀释后的金微子标记为例，加入 20uL 的 10%BSA。室温震荡反应 30 分钟
v. 离心：在 400~500g 条件下，离心 5 分钟
vi. 复溶：用复溶液将沉淀重悬至 50~150uL，进行喷垫；或者将沉淀重悬至 200~500uL，进行涂垫。
注：1.如在标记过程中，推荐 pH 条件下均出现聚沉，无需担心，后续步骤（封闭和复溶时）超声即可分散。稀释金微子时缓冲液的选择以最终满足产品性能的条件为准。
2. 建议在第一次标记时，进行最适标记 pH 值的筛选，建议用 pH6.2~pH9.0 之间的几种缓冲液，同时稀释金微子进行标记，以最终显色程度最强的条件，作为最适标记条件。
3. 标记时适当提高金微子的浓度，有助于灵敏度的提升。可将金微子稀释至 7.5 OD（188μL 金微子+312μL 缓冲液）、10 OD（250μL 金微子+250μL 缓冲液）。
注意事项
a) 贮存：本产品在室温条件下运输。收到后立即转到 2- 8°C 下储存，不可冷冻保存
b) 取用或分装时，请使用干净的容器，以免污染造成聚集，建议使用 PC 透明材质。
c) 效期：未开封 2~8℃保存 18 个月，开瓶后请在 2 个月内使用完毕。
d) 开瓶后因任何原因造成的聚集等非正常态的情况，需自行承担责任。
f）若出现添加血清样本释放不好的情况，建议在样品垫中（或者样本稀释液中）加入适量的 EDTA 钠盐。
Product Advantages
a). Simple Labeling ProcessCompared with traditional latex microspheres, Gold microsphere nanospheres feature a simpler labeling process and easier centrifugation, with a centrifugal force not exceeding 500×g, eliminating the need for additional expensive centrifugation equipment.
b). Diverse Color Options Gold microsphere offers a wide range of colors to support customized solutions for customers, while retaining the advantages of colloidal gold: ease of labeling, no color loss during centrifugation, and stable color development.
c). Higher Sensitivity Superior sensitivity compared to latex microspheres of the same particle size, and significantly better than conventional colloidal gold.
d). Excellent UniformityHigh monodispersity with PDI < 0.2.
e). Strong AdaptabilityExcellent performance even on membranes with low wicking rates.
Recommended Labeling Protocol
Taking antibody labeling as an example:
i. Dilution: Take 100 μL of stock solution, add 280 μL of water to a final volume of 380 μL.
ii. Labeling: Add the antibody (or antigen) to be labeled to the diluted Gold microsphere suspension at a final concentration of 10 μg/mL–50 μg/mL, and mix by shaking.
iii. pH Adjustment: To the Gold microsphere suspension with antibody added, it is recommended to add 20 μL of buffer (0.2 mol/L, pH range 4–10) for pH adjustment. React with shaking at room temperature for 30 minutes.
iv. Blocking: Add BSA to a final concentration of 5‰. For 400 μL of diluted Gold microsphere labeling system, add 20 μL of 10% BSA. React with shaking at room temperature for 30 minutes.
v. Centrifugation: Centrifuge at 400–500×g for 5 minutes.vi. Resuspension: Resuspend the pellet in resuspension buffer to 50–150 μL for spraying onto pads; or resuspend to 200–500 μL for coating onto pads.
*Note: 1.If aggregation occurs under the recommended pH conditions during labeling, no concern is needed – sonication during subsequent steps (blocking and resuspension) will achieve redispersion. The selection of buffer for diluting Gold microsphere shall be based on conditions that ultimately meet the product performance
2.It is recommended to screen the optimal labeling pH for the first labeling experiment. Prepare multiple buffers ranging from pH 6.2 to pH 9.0, dilute Gold microsphere respectively for labeling, and select the condition with the strongest final color development as the optimal labeling condition.
3. Appropriately increasing the concentration of Gold microsphere during labeling helps improve detection sensitivity. The microspheres can be diluted to 7.5 OD (188 μL Gold microsphere+ 312 μL buffer) and 10 OD (250 μL Gold microsphere + 250 μL buffer). requirements.
Precautions
a). Storage: This product can be transported at room temperature. Upon receipt, store immediately at 2–8°C; freezing is prohibited.
b). When sampling or aliquoting, use clean containers to avoid aggregation caused by contamination. PC transparent material is recommended.
c). Shelf Life: 18 months when stored unopened at 2–8°C. Use within 2 months after opening.
d). Any abnormal state such as aggregation caused by any reason after opening shall be the sole responsibility of the user.
e). If poor release from serum samples is observed, it is recommended to add an appropriate amount of EDTA disodium salt in the sample pad (or sample diluent).
规格
规格或纯度
20 OD, 紫色, 300nm，用于体外诊断
英文名称
Gold microsphere nanospheres
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
未知
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
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技术文档和文章
溶液计算器
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