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α-GC(EC 3.2.1.20)广泛存在于动物、植物、微生物和培养细胞中，催化水解芳基或烃基与糖基之间的α-糖苷键生成葡萄糖，不仅与细胞壁的松弛或加固有关，而且与细胞识别和一些信号分子产生密切相关。 测定原理：α-GC分解对-硝基苯-α-D吡喃葡萄糖苷生成对-硝基苯酚，后者在400nm有最大吸收峰，通过测定吸光值升高速率来计算α-GC活性。
α-GC (EC 3.2.1.20) is widely present in animals, plants, microorganisms, and cultured cells. It catalyzes the hydrolysis of α‑glycosidic bonds between aryl or alkyl groups and sugar moieties to produce glucose. It is not only involved in cell wall loosening or reinforcement but also closely related to cell recognition and the generation of certain signaling molecules. Determination principle: α‑GC cleaves p‑nitrophenyl‑α‑D‑glucopyranoside to produce p‑nitrophenol, which has a maximum absorption peak at 400 nm. The α‑GC activity is calculated by measuring the rate of increase in absorbance.
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