计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
GBSS(EC 2.4.1.21)以束缚态存在于淀粉体中,催化淀粉链的加长反应,主要负责直链淀粉的合成。 测定原理:GBSS催化ADPG与淀粉引物(葡聚糖)反应,将葡萄糖分子转移到淀粉引物上,同时生成ADP;进一步通过反应体系中添加的丙酮酸激酶、己糖激酶和6-磷酸葡萄糖脱氢酶依次催化NADP+还原为NADPH,其中NADPH生成量与前一步反应生成的ADP数量呈正比,通过340nm下测定NADPH的增加量,可以计算GBSS活性。
GBSS (EC 2.4.1.21) exists in a bound form within the amyloplast and catalyzes the elongation of starch chains, playing a primary role in amylose synthesis. Determination Principle: GBSS catalyzes the reaction between ADPG and a starch primer (glucan), transferring glucose molecules to the starch primer while generating ADP. Subsequently, pyruvate kinase, hexokinase, and glucose-6-phosphate dehydrogenase added to the reaction system sequentially catalyze the reduction of NADP⁺ to NADPH. The amount of NADPH produced is proportional to the amount of ADP generated in the preceding reaction. GBSS activity can be calculated by measuring the increase in NADPH at 340 nm.