GCDH（EC 1.1.1.47）催化D-葡萄糖和NAD(P)⁺生成D-葡萄糖酸和NAD(P)H，大量存在于高等动物的肝脏和弱氧化醋杆菌中。在低聚果糖生产中使用GCDH，不仅能去除低聚果糖中的葡萄糖提高低聚果糖的含量，而且生成的葡萄糖酸与钙离子结合生成的葡萄糖酸钙是一种理想的补钙制剂。因而，GCDH已成为制备高含量低聚果糖的理想用酶。 测定原理：GCDH催化D-葡萄糖和NAD⁺生成D-葡萄糖酸和NADH，在340nm下测定NADH上升速率，即可反映GCDH活性。
GCDH (EC 1.1.1.47) catalyzes the reaction of D-glucose and NAD(P)⁺ to produce D-gluconic acid and NAD(P)H. It is abundantly present in the liver of higher animals and in weakly oxidizing acetic acid bacteria. In the production of fructooligosaccharides, the use of GCDH not only removes glucose from fructooligosaccharides to increase their content, but also produces gluconic acid, which combines with calcium ions to form calcium gluconate, an ideal calcium supplement. Therefore, GCDH has become an ideal enzyme for the preparation of high-content fructooligosaccharides. Determination Principle: GCDH catalyzes the reaction of D-glucose and NAD⁺ to produce D-gluconic acid and NADH. GCDH activity is determined by measuring the rate of increase in NADH at 340 nm.
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