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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
乙醇酸氧化酶(EC1.1.3.15)是乙醇酸循环中的一种酶,也是植物光呼吸代谢中的关键酶,催化乙醇酸氧化生成乙醛酸,通过测定乙醇酸氧化酶活性,可以了解植物光合和呼吸代谢的基本方法。该产品提供了一种简单、方便、快速的乙醇酸氧化酶活性检测方法,适用于植物组织样本。其原理是乙醇酸氧化酶催化乙醇酸氧化生成乙醛酸,乙醛酸和盐酸苯肼反应生成乙醛酸苯腙,在 324 nm 有特征吸收峰。
Glycolate oxidase (EC 1.1.3.15) is an enzyme in the glycolate cycle and also a key enzyme in plant photorespiration metabolism. It catalyzes the oxidation of glycolate to produce glyoxylate. By measuring glycolate oxidase activity, fundamental aspects of plant photosynthesis and respiration metabolism can be understood. This product provides a simple, convenient, and rapid method for assaying glycolate oxidase activity, suitable for plant tissue samples. Its principle is that glycolate oxidase catalyzes the oxidation of glycolate to form glyoxylate. Glyoxylate then reacts with phenylhydrazine hydrochloride to produce glyoxylate phenylhydrazone, which has a characteristic absorption peak at 324 nm.