BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
L-γ-谷氨酰基转移酶(GGT或γ-GT)是催化γ-谷氨酰基移换反应的酶，γ-谷氨酰基从谷胱甘肽或其他含γ-谷氨酰基物质中转移到另一肽或氨基酸分子上，GGT主要存在于肝细胞膜和微粒体上，参与谷胱甘肽的代谢，血清中主要来自肝胆系统，当肝内合成亢进或胆汁排出受阻时血清中GGT往往容易增高。 该试剂盒以萘胺盐为底物，在GGT催化下γ-谷氨酰基转移到甘肽分子上，同时释放出游离的α-萘胺，后者与重氮盐反应，产生红色化合物，其颜色深浅与GGT浓度呈正比，通过酶标仪检测530nm处吸光度，进而计算酶的活性。本产品仅用于科研领域，不适用于临床诊断或其他用途。
L-γ-glutamyltransferase (GGT or γ-GT) is an enzyme that catalyzes the transfer of γ-glutamyl groups. It transfers the γ-glutamyl group from glutathione or other substances containing γ-glutamyl groups to another peptide or amino acid molecule. GGT is primarily located on the membranes of hepatocytes and in microsomes, participating in the metabolism of glutathione. In serum, GGT mainly originates from the hepatobiliary system. When hepatic synthesis is亢进 (enhanced/increased) or when bile excretion is obstructed, serum GGT levels tend to increase. The detection principle of this kit is as follows: Using a naphthylamine salt as a substrate, the γ-glutamyl group is transferred to a peptide molecule catalyzed by GGT, simultaneously releasing free α-naphthylamine. The α-naphthylamine then reacts with a diazonium salt to produce a red compound. The intensity of the color is proportional to the GGT concentration. The absorbance is measured at 530 nm using a microplate reader, and the enzyme activity is subsequently calculated. This product is intended for research use only and is not suitable for clinical diagnostic or other purposes.
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