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3-磷酸甘油(G3P)被3-磷酸甘油氧化酶(GPO)氧化生成过氧化氢(H₂O₂),H₂O₂与4-氨基氨替吡啉等反应生成红色醌类化合物,其在510nm处有特征吸收峰,通过检测510nm处吸光值变化即可得出3-磷酸甘油氧化酶(GPO)活性大小。
Glycerol-3-phosphate (G3P) is oxidized by glycerol-3-phosphate oxidase (GPO) to produce hydrogen peroxide (H₂O₂). H₂O₂ then reacts with 4-aminoantipyrine and other components to form a red quinone compound, which exhibits a characteristic absorption peak at 510 nm. The activity of glycerol-3-phosphate oxidase (GPO) is determined by measuring the change in absorbance at this wavelength.