γ-氨基丁酸转氨酶(GABA-T，EC 2.6.1.96)是GABA支路中的关键酶之一，催化GABA的降解和转化。本试剂盒利用γ-氨基丁酸转氨酶（GABA-T）催化丙酮酸和GABA反应生成生成琥珀酸半醛和丙氨酸，通过检测GABA的减少量进而得出GABA-T酶活力大小。反应方程式：4-aminobutanoate + pyruvate = succinate semialdehyde + L-alanine

γ-Aminobutyrate transaminase (GABA-T, EC 2.6.1.96) is one of the key enzymes in the GABA shunt, catalyzing the degradation and conversion of GABA. This kit utilizes γ-aminobutyrate transaminase (GABA-T) to catalyze the reaction between pyruvate and GABA, producing succinate semialdehyde and alanine. The activity of GABA-T is determined by measuring the decrease in GABA content. Reaction equation: 4-aminobutanoate + pyruvate = succinate semialdehyde + L-alanine

规格

规格或纯度

BioReagent

英文名称

GABA Transaminase Activity Assay Kit (Colorimetric Method)

应用

酶活性检测

检测方法

比色法

检测仪器

可见光分光光度计

检测波长

645nm

储存与运输

储存条件

2-8°C储存,避光

运输条件

低温运输

稳定性与储存

避光，保存于4℃，保质期3个月。

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

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TCA循环谷氨酸微量法氨基酸代谢氮同化比色法同位素示踪代谢重编程 LC-MS/MS α-酮戊二酸碳氮代谢谷氨酰胺转氨反应

Mechanisms of Carbon-Nitrogen Metabolic Crosstalk: Glutamate, α-Ketoglutarate, and the Amino Acid Metabolic Network

目录：技术文章

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TCA cycle Glutamate amino acid metabolism Nitrogen assimilation isotope tracing Metabolic Reprogramming LC-MS/MS α-ketoglutarate Colorimetric Method Carbon-Nitrogen Metabolism Glutamine Transamination Micro Method

溶液计算器