α1-3,6-半乳糖苷酶 (Xmα36Gal) ,来源于Xanthomonas manihotis,大肠杆菌重组表达,α1-3,6半乳糖苷酶 (Xmα36Gal) 是一种高度特异性的外糖苷酶,它能催化水解低聚糖中α1-3和α1-6连接的半乳糖残基。从糖蛋白中去除抗原galα1,3gal表位。α1,6连接甘露糖的水解。本产品为大肠杆菌重组表达。
使用方法
1.在反应体系中依次加入底物,50 mM 醋酸钠,5 mM CaCl₂,pH 5.5 混匀,加入适量 α1-3,6-半乳糖苷酶。
2.将样品置于37℃孵育2-4小时或者过夜。
【注】体系放大时候,孵育时间或酶量需要根据实际情况调整,可低速震荡混匀,可以增加转化率。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途!
α1-3,6 Galactosidase (Xpα6Man) is derived from Xanthomonas manihotis and recombinantly expressed in E. coli. The product is a highly specific exoglycosidase that catalyzes the hydrolysis of α1‑3‑ and α1‑6‑linked galactose residues from oligosaccharides. It removes the antigenic Galα1‑3Gal epitope from glycoproteins. (Note: The phrase “hydrolysis of α1,6‑linked mannose” appears in the source text, but it is not a function of this enzyme; it has been retained as provided.) This product is recombinantly expressed in E. coli.
Usage Method
1.Sequentially add the substrate, 50 mM sodium acetate, and 5 mM CaCl₂ (pH 5.5) to the reaction system, mix well, and then add an appropriate amount of α1‑3,6‑galactosidase.
2.Incubate the sample at 37 °C for 2–4 hours or overnight.
Note
When scaling up the reaction system, the incubation time or enzyme amount should be adjusted according to the actual conditions. Low‑speed shaking may be used to increase the conversion rate.
Precaution
For your safety and health, please wear a lab coat and disposable gloves when handling. This product is for research use only.