计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于酵母 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
酵母菌属单细胞的真核微生物,其细胞核与细胞质有明显分化。酵母细胞一般呈卵圆形、圆形、圆柱形或柠檬形。每种酵母菌细胞有其一定的形态大小。观察酵母菌个体形态时,应注意其细胞形状。酵母菌的细胞质中含有一个或几个透明的“小液滴”,即液泡。处于旺盛生长阶段的酵母菌,液泡中没有内含物,老化细胞的液泡中出现了脂肪滴和肝糖粒等颗粒状贮藏物,可利用中性红将液泡染成红色,利用苏丹黑将脂肪粒氧化成蓝黑色,利用碘液将肝糖粒染成深红褐色,从而在光学显微镜下可观察到酵母菌细胞中存在着上述等特殊结构。
肝糖粒染色液 (1%) 主要由碘化钾和 1% 碘组成,主要用于酵母涂片的染色,酵母菌中的肝糖粒被染成深红色或红褐色。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤 (仅供参考):
1、将酿酒酵母涂片,自然干燥。
2、滴加 1~2 滴本染色液至涂片上,染色 1~2min。
3、加盖玻片,显微镜下观察染色效果。
注意事项:
1、涂片之前应事先在背面做好圆圈标记,以便判断后续试验的位置。
2、本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Yeasts are unicellular eukaryotic microorganisms with distinct differentiation between cell nucleus and cytoplasm. Yeast cells are generally oval, spherical, cylindrical or lemon-shaped, and each strain has a specific morphological size. Attention should be paid to cell morphology when observing individual yeast cells.One or several transparent tiny droplets known as vacuoles exist in the cytoplasm of yeast cells. Vacuoles of vigorously growing yeast contain no inclusions, while vacuoles in aged cells accumulate granular storage substances such as fat droplets and glycogen granules. Vacuoles can be stained red with neutral red; fat droplets can be stained blue-black with Sudan Black; glycogen granules can be stained dark reddish-brown with iodine solution. These special structures inside yeast cells can then be observed under an optical microscope.
1% Glycogen Granule Staining Solution is mainly composed of potassium iodide and 1% iodine. It is applied to stain yeast smears, staining glycogen granules inside yeast cells deep red or reddish-brown. This reagent is intended solely for scientific research and shall not be used for clinical diagnosis or other purposes.
Operating Procedures (For Reference Only):
1. Prepare a smear of Saccharomyces cerevisiae and allow it to air-dry naturally.
2. Drop 1~2 drops of this staining solution onto the smear and stain for 1~2 minutes.
3. Place a coverslip over the sample and observe the staining result under a microscope.
Precautions:
1. Mark a circle on the reverse side of the slide before preparing the smear to locate the observation area in follow-up experiments.
2. This product is restricted to scientific research performed by professional personnel. It must not be used for clinical diagnosis or treatment, food or pharmaceutical production, nor stored in residential premises.
3. Wear a lab coat and disposable gloves during operation to ensure personal safety and health.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | G1525447 | |
| 分析证书 | G1525447 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for Yeasts 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →