计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
全世界植物约 50 万种,分布广泛,多种多样,形态各异,在植物学教学、科学研究、陈列展览、开发利用和植物资源保护等方面,为了要对新鲜花朵、肉质果实及地下根茎等进行长期保存,必须将材料浸泡在药液中,制成浸制标本,也可以做成蜡叶标本、木材标本、果实种子标本、立体标本、形态解剖标本和叶脉标本等形式的标本。各种形式的标本是根据不同的需要和要求制作出来的,各自有专门的用途,有些植物标本不仅用在教学和科研上,而且还可用来观赏或者制作成过塑的保绿蜡叶标本,既可作为装饰工艺品,又作为山区风景旅游区的纪念品,别具特色。
绿色标本保色液 (快速) 的作用原理是在加热的条件下,保色液中的铜离子可置换绿色植物叶片叶绿素中的镁离子,使其变成保绿标本。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤 (仅供参考):
1、将绿色标本保色液 (快速) 加水稀释 3~5 倍,加热升温至 70~80℃。
2、采集的绿色植物叶片洗净晾干,投入上述保色液中,并不时翻动叶片,10~30 分钟左右,标本的绿色消失后又重新恢复。
3、待绿色稳定后取出标本,用蒸馏水清洗 2 次,去除标本表面的保色液。
4、放入 5%~6% 的福尔马林溶液中可长期保存。
5、也可制成蜡叶标本。方法仅供参考:捞出用蒸馏水清洗的标本晾干,用吸水纸压干,装订成造型美观的标本,并用升汞消毒处理 (升汞剧毒,操作需在通风橱内进行),最后用过塑机将整个标本用塑封膜密封起来。
注意事项:
1、保色处理的时间需根据标本大小等因素而定,需确保标本颜色由绿色消失至无色,再恢复绿色,而且绿色稳定,才能结束操作。 2、保色液的稀释比例可以根据预实验的情况,找到最合适的稀释比例。
3、废弃的保色液,可能还有铜离子残留,请按规定合理处理。
4、为保障安全与健康,请穿实验服、佩戴一次性手套进行操作。
5、试剂开封后请尽快使用,避免影响后续实验效果。
There are approximately 500,000 plant species worldwide, widely distributed with diverse and varied morphologies. For botanical teaching, scientific research, exhibition display, development and utilization, and plant resource conservation, fresh flowers, fleshy fruits, underground rhizomes and other materials require long-term preservation. Materials must be immersed in chemical solution to prepare liquid-preserved specimens. Other specimen types can also be prepared, including herbarium sheets, wood specimens, fruit and seed specimens, three-dimensional specimens, morphological anatomical specimens and leaf vein specimens. Specimens of various types are prepared according to different requirements, each with dedicated applications. Some plant specimens are not only used for teaching and scientific research, but also for ornamental purposes, or processed into plastic-coated green-preserved herbarium sheets. These specimens can serve as decorative crafts as well as souvenirs for mountain scenic spots with distinctive features.
Working Principle of Green Specimen Color Preservation Solution (Rapid): Under heating conditions, copper ions in the preservation solution replace magnesium ions in chlorophyll of green plant leaves to form green-preserved specimens. This reagent is for research use only and is not intended for clinical diagnosis or other applications.
Operating Procedure (For Reference Only):
1. Dilute Green Specimen Color Preservation Solution (Rapid) with water at 3–5 fold, heat to 70–80℃.
2. Collect green plant leaves, wash and air-dry them, immerse in the above preservation solution and flip the leaves from time to time for about 10–30 minutes until the original green color of the specimen fades and recovers again.
3. Take out the specimen once the green color stabilizes, rinse it twice with distilled water to remove residual preservation solution on the specimen surface.
4. Transfer the specimen into 5%–6% formalin solution for long-term storage.
5. Herbarium sheets can also be prepared. Method for reference: Take out the distilled-water-rinsed specimen and air-dry it; press and dry the specimen with blotting paper, then bind it into neatly shaped specimens. Sterilize the specimens with mercuric chloride (mercuric chloride is highly toxic; operations must be performed in a fume hood). Finally, use a laminator and plastic film to fully seal the specimen.
Precautions:
1. The duration of color preservation treatment varies depending on specimen size and other factors. The treatment can only be terminated after observing that the green color of the specimen fades to colorless, redevelops and remains stable.
2. The dilution ratio of preservation solution can be optimized according to preliminary tests.
3. Waste preservation solution contains residual copper ions and shall be disposed of properly in accordance with hazardous waste regulations.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
5. Use the reagent as soon as possible after opening to avoid compromising subsequent experimental results.