通用定影液是用于 Western、Northern、Southern 等后续压片曝光后显影的定影溶液。配方特别适用 X 光片的定影，也可用于普通的黑白底片或照片的定影，通用显影粉是通用定影液的粉剂状态，溶解于去离子水后即为通用定影液。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料
1、X 光片、红灯、磁力搅拌器、烧杯
2、自来水、去离子水、显影液
操作步骤 (含显影定影，仅供参考)
1、配制通用定影液：取 1 升烧杯，加入约 800ml 的去离子水中（25℃左右），加入通用定影粉，充分溶解后再补水至 1000ml，即可使用。如有必要可用 100 目滤网过滤一次。
2、显影：把 X 光片完全浸没在显影液内，一般显影 10s～2min。具体显影时间要根据显影情况而定，可以在红灯下观察，当发现显影的程度已经达到预定目标，即可停止显影，进行后续步骤。
3、水洗：在自来水中冲洗 2～3min，以完全冲洗去显影液。
4、定影：浸没在定影液内，定影 2～5min 左右，定影时间再长一些如 10min，通常对洗片的结果没有明显影响。
5、水洗：在自来水中冲洗 2～5min，以充分洗去各种残留溶液和试剂。
注意事项
1、曝光、显影、定影步骤均应在严密的避光环境中进行。
2、显影液用于胶片时：冲卷 2~6 分钟，印放 1~3 分钟。
3、定影液用于胶片时：软片约 10 分钟，印放 10~15 分钟。
4、试剂可重复使用 5~10 次，不宜超过 20 次，最终重复次数需根据使用效果酌情考量。
5、试剂配成溶液后，应 4℃避光保存，6 个月有效。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
7、试剂开封后请尽快使用，以防影响后续实验效果。
Universal Fixing Solution is a fixing solution used for film processing after exposure in Western, Northern, Southern blot and other related experiments. This formula is specially optimized for X-ray film fixing, and it can also be applied to conventional black-and-white negatives and photographic paper. Universal Fixing Powder is the powdered form of Universal Fixing Solution; it can be prepared into working fixing solution after dissolving in deionized water. This reagent is intended for research use only and shall not be applied to clinical diagnosis or other purposes.
Materials to Be Prepared by User
1. X-ray films, red safe light, magnetic stirrer, beaker
2. Tap water, deionized water, developing solution
Operating Procedures (Including Development & Fixation, For Reference Only)
1. Prepare Universal Fixing Solution: Add approximately 800 mL deionized water (around 25℃) into a 1 L beaker, add the entire pack of Universal Fixing Powder and stir thoroughly until fully dissolved, then top up with deionized water to 1000 mL for use. Filtration with a 100-mesh filter screen is optional if necessary.
2. Development: Fully immerse the X-ray film in developing solution for 10 seconds to 2 minutes generally. Adjust the developing time according to the actual developing effect. Observe under red safe light and terminate development once the expected band intensity is achieved, then proceed to the next steps.
3. Rinsing: Rinse the film with tap water for 2-3 minutes to completely remove residual developing solution.
4. Fixation: Submerge the film in fixing solution for about 2-5 minutes. Extending the fixation time to 10 minutes will not produce obvious adverse effects on the final result.
5. Final Washing: Rinse thoroughly with tap water for 2-5 minutes to eliminate residual reagents.
Precautions
1. All procedures including exposure, development and fixation must be performed in a strictly light-proof environment.
2. For film development: 2-6 minutes for roll film developing, 1-3 minutes for print making.
3. For film fixation: around 10 minutes for negative film, 10-15 minutes for print making.
4. The working solution can be reused for 5-10 times, with a maximum reuse limit of 20 times; adjust the service life based on practical performance.
5. After preparation into liquid form, store the solution away from light at 4℃, valid for 6 months.
6. Wear a lab coat and disposable gloves during operation for personal safety and health.
7. Use the reagent as soon as possible after opening the package to avoid compromised experimental performance.
ZJ26F0838633
危险品化学品经营许可证（带存储）