计算溶液所需的质量、体积或浓度。
| 活性类型 | 活性值-log(M) | 作用机制 | 期刊 | 参考文献(PubMed IDs) |
|---|
血小板膜糖蛋白IIb/IIIa (GPIIbIIIa) 来源于人血小板
有货
为什么选择此级别
BioReagent,天然 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
血小板膜糖蛋白参与血小板黏附与聚集过程。糖蛋白 IIb 和 IIIa（GPIIb 与 GPIIIa）共同构成纤维蛋白原受体，是血小板聚集所必需的蛋白。
阿拉丁提供纯化自人血小板的 GPIIbIIIa。 糖蛋白 IIb 包含 2 个二硫键相连的亚基：GPIIb（分子量 = 125000）与 GPII（分子量 = 23000）；GPIIIa 仅含单条多肽链（分子量 = 108000）。 GPIIbIIIa 凝胶电泳表观分子量如下：GPIIb 在非还原条件下为 136000，还原条件下为 125000；GPIIIa 非还原条件下 97000，还原条件下 108000。蛋白浓度采用 Bradford 法测定。百分消光系数（1%）=9.1。
该蛋白可识别 RGD 肽底物。
本蛋白纯化自人血小板，原料供体采用 FDA 批准的检测方法检测：抗 HBc 抗体、HCV 抗体、HIV 1/2 抗体、HTLV‑I/II 抗体均为阴性；HBsAg、HIV-1 RNA、HCV RNA、HBV DNA、梅毒检测均无反应性。供体同时进行克雅病（CJD）筛查。
Platelet membrane glycoproteins are involved in platelet adhesion and aggregation. Glycoproteins IIb and IIIa (GPIIb and GPIIIa) constitute the fibrinogen receptor and are required for platelet aggregation.
Aladdin offers GPIIbIIIa purified from human platelets. Glycoprotein IIb consists of 2 disulfide-linked subunits GPIIb (MW = 125,000) and GPII (MW= 23,000) while GPIIIa has only one polypeptide chain (MW= 108,000). GPIIbIIIa migrates on gels as follows: GPIIb 136,000 non-reduced and 125,000 reduced. GPIIIa is 97,000 non-reduced and 108,000 reduced. Protein concentration is determined via the Bradford method. Extinction Coefficient (1%) = 9.1 The GPIIbIIIa migrates on gels as follows: GPIIb 136,000 non-reduced and 125,000 reduced. GPIIIa is 97,000 non-reduced and 108,000 reduced. The protein does recognize RGD peptide substrate.
The above protein was purified from Human platelets that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.
规格
产品名称
血小板膜糖蛋白IIb/IIIa (GPIIbIIIa) 来源于人血小板
规格或纯度
BioReagent, 天然, 蛋白浓度见COA
生化机理
GPIIb: Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface (By similarity). Integrin ITGA2B:ITGB3 is also the receptor of erythrocyte-specific ICAM4 ligand involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets. GPIIIa: Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor (VWF). Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling. ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling. ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV:ITGB3 act as a receptor for CD40LG. ITGAV:ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP. Integrin ITGA2B:ITGB3 is also the receptor of the erythrocyte-specific ICAM4 ligand involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets. ITGAV:ITGB3 acts as a receptor for SNED1 and mediates R-G-D-dependent cell adhesion to SNED1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Coxsackievirus A9. (Microbial infection). Acts as a receptor for Hantaan virus. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Cytomegalovirus/HHV-5. (Microbial infection) Integrin ITGA5:ITGB3 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGAV:ITGB3 acts aP05556s a receptor for Human parechovirus 1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Post-translational: GPIIb: Integrin alpha-IIb heavy chain: Cleaved by ELANE; the cleavage promotes activation of platelet fibrinogen receptor integrin alpha-IIb/beta-3.
种属
人(Human)
来源
天然
预测分子量
GPIIb: 136 kDa non-reduced, 125 kDa reduced. GPIIIa: 97 kDa non-reduced, 108 kDa reduced.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
20 mM Tris-HCl, 0.1 M NaCl, 1 mM CaCl₂, 0.05% NaN₃, 50% Glycerol, pH 7.4
防腐剂
0.05% Sodium azide
浓度
蛋白浓度见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃；收货后建议分装，避免反复冻融。
技术文档
高级数据
关联靶点（人）
ITGA2B
Tclin
整合素α-IIb(Integrin alpha-IIb)
(0 活性数据)
ITGB3
Tclin
整合素β-3(Integrin beta-3)
(0 活性数据)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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溶液计算器
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