BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
γ-谷氨酸激酶（γ-GK，EC 2.7.2.11）是脯氨酸生物合成途径中的关键酶之一，催化谷氨酸生成脯氨酸途径的第一步反应，在植物渗透调节和逆境适应中发挥重要作用。本试剂盒采用羟肟酸–铁络合比色法测定γ-GK活性：γ-GK催化谷氨酸磷酸化生成L-谷氨酸5-磷酸，后者在羟胺存在下转化为γ-谷氨酰基异羟肟酸；该产物在酸性条件下与Fe³⁺螯合，形成在535 nm处具有特征吸收峰的Hydroxamate-Fe³⁺复合物。通过测定该波长下吸光值的增幅，即可精准计算样品中γ-GK的酶活力大小。
γ-Glutamyl kinase (γ-GK, EC 2.7.2.11) is one of the key enzymes in the proline biosynthesis pathway, catalyzing the first step in the conversion of glutamate to proline, and plays an important role in plant osmotic regulation and stress adaptation. This kit employs a hydroxamic acid–iron complexation colorimetric method for the determination of γ-GK activity: γ-GK catalyzes the phosphorylation of glutamate to L-glutamate 5-phosphate, which is subsequently converted to γ-glutamyl hydroxamate in the presence of hydroxylamine. This product chelates with Fe³⁺ under acidic conditions to form a Hydroxamate-Fe³⁺ complex with a characteristic absorbance peak at 535 nm. By measuring the increase in absorbance at this wavelength, the γ-GK enzyme activity in the sample can be precisely calculated.
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