BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
甘油是脂肪细胞中甘油三酯代谢的终产物之一，在工业与生活中亦广泛用作溶剂、润滑剂、药剂和甜味剂。 其含量测定原理如下：甘油在甘油激酶（GK）催化下生成甘油-1-磷酸（G-1-P）；后者经甘油磷酸氧化酶（GPO）氧化，产生过氧化氢（H₂O₂）。生成的H₂O₂与4‑氨基安替比啉及色原物质反应，形成红色醌类化合物。该产物在510 nm波长处具有特征吸收峰，通过测定该波长下的吸光度值，即可定量计算样品中的甘油含量。
Glycerol is one of the end products of triglyceride metabolism in adipocytes, and is also widely used as a solvent, lubricant, pharmaceutical excipient, and sweetener in industry and daily life. The determination principle is as follows: Glycerol is converted to glycerol-1-phosphate (G-1-P) by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to produce hydrogen peroxide (H₂O₂). The generated H₂O₂ reacts with 4-aminoantipyrine and a chromogenic substrate to form a red quinone compound, which exhibits a characteristic absorbance peak at 510 nm. By measuring the absorbance at this wavelength, the glycerol content in the sample can be quantitatively calculated.
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