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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
谷氨酸广泛存在于动物、植物、微生物及培养细胞中，不仅是构成蛋白质的20种常见氨基酸之一，也是细胞代谢中的关键分子。此外，谷氨酸作为哺乳动物神经系统中最丰富的快速兴奋性神经递质，同时广泛用于食品工业，用作增味剂。 本试剂盒的检测原理如下：谷氨酸脱氢酶（GLDH）特异性催化底物谷氨酸，同时将NAD⁺还原为NADH。NADH在340 nm波长处具有特征吸收峰，通过测定该波长下吸光度的增加量，即可定量计算样品中的谷氨酸含量。
Glutamate is widely present in animals, plants, microorganisms, and cultured cells. It is not only one of the 20 common amino acids that constitute proteins, but also a key molecule in cellular metabolism. In addition, glutamate serves as the most abundant fast excitatory neurotransmitter in the mammalian nervous system and is widely used in the food industry as a flavor enhancer. The detection principle of this kit is as follows: Glutamate dehydrogenase (GLDH) specifically catalyzes the substrate glutamate, while reducing NAD⁺ to NADH. NADH exhibits a characteristic absorbance peak at 340 nm. By measuring the increase in absorbance at this wavelength, the glutamate content in the sample can be quantitatively calculated.
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