BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
谷氨酸广泛存在于动物、植物、微生物及培养细胞中，不仅是组成蛋白质的20种氨基酸之一，也是细胞代谢中的关键分子。此外，谷氨酸不仅是哺乳动物神经系统中最丰富的快速兴奋性神经递质，还存在于多种食品中，并作为增味剂应用于食品工业。本试剂盒提供一种快速、灵敏的谷氨酸检测方法：谷氨酸在谷氨酸脱氢酶（GDH）特异性催化下脱氢，生成的NADH进一步与显色剂反应生成在450 nm处具有特征吸收峰的黄色产物，通过测定该波长下吸光值的增幅，结合标准曲线即可精准计算样品中谷氨酸的含量。
Glutamate is widely present in animals, plants, microorganisms, and cultured cells. It is not only one of the 20 amino acids that constitute proteins, but also a key molecule in cellular metabolism. In addition, glutamate serves as the most abundant fast excitatory neurotransmitter in the mammalian nervous system, and is also found in various foods and used as a flavor enhancer in the food industry. This kit provides a rapid and sensitive method for the determination of glutamate: Glutamate is specifically dehydrogenated by glutamate dehydrogenase (GDH), and the generated NADH further reacts with a chromogenic reagent to form a yellow product with a characteristic absorbance peak at 450 nm. By measuring the increase in absorbance at this wavelength and referencing a standard curve, the glutamate content in the sample can be precisely calculated.
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