BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
谷氨酸合酶（GOGAT）分布于植物中，与谷氨酰胺合成酶（GS）共同构成GS/GOGAT循环，参与氨同化的调控。本试剂盒的测定原理为：GOGAT催化谷氨酰胺的氨基转移至α-酮戊二酸，生成两分子谷氨酸；同时，NADH被氧化为NAD⁺，导致340 nm处吸光度下降。通过测定340 nm处吸光度的下降速率，即可计算GOGAT的酶活性。
Glutamate synthase (GOGAT) is distributed in plants and, together with glutamine synthetase (GS), constitutes the GS/GOGAT cycle, participating in the regulation of ammonia assimilation. The assay principle of this kit is as follows: GOGAT catalyzes the transfer of the amino group from glutamine to α-ketoglutarate to generate two molecules of glutamate. Simultaneously, NADH is oxidized to NAD⁺, resulting in a decrease in absorbance at 340 nm. By measuring the rate of decrease in absorbance at 340 nm, the GOGAT enzyme activity can be calculated.
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