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葡萄糖-6-磷酸酶（glucose 6 phosphatase, G6Pase, EC 3.1.3.9）广泛存在于动物、植物、微生物及细胞中，是糖异生过程中催化葡萄糖-6-磷酸水解为葡萄糖的限制酶，在维持血糖动态平衡方面发挥重要作用。本试剂盒提供了一种简便的检测方法，适用于动物组织、植物组织、细胞、血清（浆）等生物样本中G6Pase活性的测定。检测原理为：G6Pase催化葡萄糖-6-磷酸生成葡萄糖；葡萄糖在葡萄糖氧化酶作用下生成过氧化氢；过氧化氢通过Trinder反应最终生成醌亚胺化合物。该化合物在554 nm处具有特征吸收峰，通过测定吸光度的变化速率即可反映G6Pase活性。
Glucose‑6‑phosphatase (G6Pase, EC 3.1.3.9) is widely distributed in animals, plants, microorganisms, and cells. It is the rate‑limiting enzyme that catalyzes the hydrolysis of glucose‑6‑phosphate to glucose in the gluconeogenesis pathway, and plays a vital role in maintaining blood glucose homeostasis. This kit provides a convenient assay method suitable for the determination of G6Pase activity in various biological samples, including animal tissues, plant tissues, cells, serum, and plasma. The assay principle is as follows: G6Pase catalyzes the hydrolysis of glucose‑6‑phosphate to glucose. The generated glucose is then oxidized by glucose oxidase to produce hydrogen peroxide (H₂O₂). The H₂O₂ subsequently reacts through the Trinder reaction to generate a quinone imine compound, which exhibits a characteristic absorption peak at 554 nm. By measuring the rate of change in absorbance, the G6Pase activity can be reflected.
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