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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
葡萄糖激酶（glucokinase, GCK）是糖代谢途径中的关键酶，参与糖酵解的第一步反应，催化葡萄糖磷酸化，促进胰岛素分泌和葡萄糖代谢，从而有效维持体内血糖平衡。GCK又称己糖激酶Ⅳ，主要在肝细胞和胰岛β细胞中表达。与其他己糖激酶相比，GCK对葡萄糖的亲和力较低（高Km）。因此，在高浓度葡萄糖条件下测得的为总己糖激酶活性，在低浓度葡萄糖条件下测得的为对葡萄糖具有高亲和性的己糖激酶（如HKⅠ、Ⅱ、Ⅲ）活性，两者之差即为GCK活性。 本试剂盒的检测原理为：GCK催化底物反应生成NADPH；NADPH将WST-8还原生成橙黄色的甲臜（formazan），该产物在450 nm附近具有最大吸收峰。通过测定450 nm处吸光度的增加速率，即可计算样本中GCK活性。
Glucokinase (GCK) is a key enzyme in the glucose metabolic pathway, participating in the first step of glycolysis by catalyzing the phosphorylation of glucose, which promotes insulin secretion and glucose metabolism, thereby effectively maintaining blood glucose homeostasis in vivo. GCK, also known as hexokinase IV, is primarily expressed in hepatocytes and pancreatic β‑cells. Compared to other hexokinases, GCK has a lower affinity for glucose (high Km). Therefore, activity measured under high glucose concentrations represents total hexokinase activity, while activity measured under low glucose concentrations represents high‑affinity hexokinases (such as HK I, II, and III). The difference between these two values corresponds to GCK activity. The assay principle of this kit is as follows: GCK catalyzes the substrate reaction to generate NADPH. NADPH then reduces WST‑8 to produce an orange‑yellow formazan, which exhibits a maximum absorption peak near 450 nm. By measuring the rate of increase in absorbance at 450 nm, the GCK activity in the sample can be calculated.
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