计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒采用NADH偶联显色法快速、灵敏地测定葡萄糖脱氢酶(GCDH)活力。相较于传统340 nm直接测定法(灵敏度低、干扰大),本方法利用GCDH催化产生的NADH与显色剂反应,生成450 nm处有特征吸收的有色产物,通过检测该产物的生成速率(与酶活力成正比),即可高效计算GCDH活性。
This kit employs an NADH‑coupled chromogenic method for the rapid and sensitive determination of glucose dehydrogenase (GCDH) activity. Compared with the traditional direct measurement at 340 nm (which suffers from low sensitivity and high interference), this method utilizes the NADH generated by GCDH catalysis to react with a chromogenic reagent, producing a colored product with a characteristic absorption peak at 450 nm. By measuring the rate of formation of this product—which is directly proportional to the enzyme activity—the GCDH activity can be efficiently calculated.