明胶粘贴剂又称为 Haupt 粘贴剂 (赫伯特粘贴剂), 常用于载玻片上切片粘附，是组织切片常用粘附试剂。主要由明胶、甘油、苯酚配制而成，适用于石蜡切片、冰冻切片及真菌子实体切片的粘附。组织切片完成时，显微镜检确认切片合格后，使用粘贴剂将切片牢固粘附于载玻片上，随后熔去石蜡 (冰冻切片无需此步骤)，再进行染色、脱水等后续操作。 经本品处理制备的载玻片，4℃ 条件下可保存半年。本试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考)
1、低温环境下明胶粘贴剂易胶冻凝固，需置于 25-37℃ 水浴加温溶解后使用。
2、滴加明胶粘贴剂至载玻片表面，静置适当时间 (也可采用浸蘸方式包被玻片)。
3、取出载玻片，37℃ 干燥 1-2h，直至肉眼观察完全干燥；也可置于通风橱内吹风数分钟加速干燥；部分实验需要干燥 3h 或更长时间。
注意事项
1、明胶可被部分细胞消化吸收，过量明胶存在一定细胞毒性。
2、采用浸蘸法包被时，玻片需完全浸没于液体中，防止包被不完全，引发样本脱落。
3、干燥过程避免尘埃污染。
4、为保障安全，请穿戴实验服、一次性手套进行操作。
5、试剂开封后建议尽快使用，以免影响后续实验效果。
Gelatin Adhesive, also known as Haupt’s Adhesive, is a common histological adhesive for attaching sections to glass slides. It is composed of gelatin, glycerol and phenol, and is suitable for adhering paraffin sections, frozen sections and fungal fruiting‑body sections. After sectioning, confirm the section quality by microscopic examination, then use this adhesive to firmly attach sections onto glass slides. Paraffin is then removed (this step is not required for frozen sections), followed by subsequent procedures such as staining and dehydration. Slides prepared with this reagent can be stored at 4 ℃ for six months. For research use only; not intended for clinical diagnosis or other applications.
Procedure (For reference only)
1. Gelatin adhesive tends to gelatinize at low temperature. Warm it in a water‑bath at 25‑37 ℃ until fully dissolved before use.
2. Apply gelatin adhesive onto the surface of glass slides and allow it to stand for an appropriate period of time (slides may also be coated by immersion).
3. Take out the slides and dry at 37 ℃ for 1‑2 h until visually completely dry. Drying can be accelerated by placing slides in a fume hood for several minutes. For some experiments, a drying time of 3 h or longer is required.
Precautions
1. Gelatin can be digested and absorbed by some types of cells; excessive gelatin may cause cytotoxicity.
2. When coating slides by immersion, ensure slides are fully submerged in the liquid. Incomplete coating may result in sample detachment.
3. Avoid dust contamination during the drying process.
4. For personal safety, wear a lab coat and disposable gloves during handling.
5. It is recommended to use up the reagent promptly after opening to prevent compromised experimental performance.
危险品化学品经营许可证（带存储）