计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
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Product Description
Our Glycan Sequencing Kit includes the enzymes and buffer required to sequence ten N-linked oligosaccharides.
Contents
Neuraminidase from Arthrobacter ureafaciens – 80 µl
Beta-Galactosidase from Streptococcus pneumoniae – 60 µl
N-Acetylglucosaminidase from Streptococcus pneumoniae) – 40 µl
Alpha-Mannosidase from Jack Bean – 20 µl
Core Alpha-Mannosidase from X. manihotis) – 10 µl
5X Reaction buffer – 400 µl
Analysis
Many methods of analysis are available, including HPLC, gel electrophoresis, HPAEC, capillary electrophoresis, and mass spectrometry. For more information on these methods, please contact us.
Stability
The Glycan Sequencing Kit is stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
Purity
All Enzymes are tested for contaminating protease by incubating 10 μg of denatured BSA with 2 μl of enzyme at 37°C for 24 hours. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
The production host strains for our recombinant enzymes have been extensively tested and do not produce any detectable glycosidases. Enzymes purified from native sources are tested for contaminating exoglycosidases The absence of exoglycosidase contaminants is confirmed by extended incubations with the corresponding pNP-glycosides.