D2511425计算溶液所需的质量、体积或浓度。
GVBE (GVB° with EDTA)
有货
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
General Description
GVBE is an isotonic buffer used in complement assays to prevent complement activation or to stop ongoing complement activation when added at the end of an incubation (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)).
Buffer Components
EDTA is used in this buffer to chelate calcium and magnesium and thereby inhibit complement activation by all three pathways. Veronal is used as the buffer because in the mid-1900s this was the only buffer for pH range 7.2-7.4 that did not chelate metal ions and did not inhibit complement reactions as did other buffers. Sodium chloride is present to provide an isotonic environment so that cells do not lyse due to osmotic pressure. Gelatin is present to prevent loss of protein components due to adsorption onto tips or tubes during dilutions and in the assays themselves. Azide is present to prevent bacterial growth.
Physical Characteristics
The concentration of gelatin in this buffer is below the concentration that forms solid gels. However, because of the gelatin is close to its gelling concentration at 4℃ some strings of gelatin form during standing at this temperature. They can be redissolved easily by heating to 37℃ or by brief heating in a microwave oven.
The concentration of gelatin in this buffer is below the concentration that forms solid gels. However, because of the gelatin is close to its gelling concentration at 4℃ some strings of gelatin form during standing at this temperature. They can be redissolved easily by heating to 37℃ or by brief heating in a microwave oven.
Applications
GVBE is often used as the buffer added at the end of an assay to stop complement activation and dilute the reaction to levels that can be read spectrophotometrically. It is also used in setting up control assays where no complement activation should occur (background control assays).
GVBE is often used as the buffer added at the end of an assay to stop complement activation and dilute the reaction to levels that can be read spectrophotometrically. It is also used in setting up control assays where no complement activation should occur (background control assays).
规格
产品名称
GVBE (GVB° with EDTA)
别名
GVBE (含 EDTA 的 GVB°)
规格或纯度
0.1 % gelatin, 5 mM Veronal, 145 mM NaCl, 0.025 % NaN3, 10 mM EDTA, pH 7.3.
分子类型
小分子
储存与运输
储存缓冲液
Sodium veronal
浓度
0.1 % gelatin, 5 mM Veronal, 145 mM NaCl, 0.025 % NaN3, 10 mM EDTA, pH 7.3.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
技术文档
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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