J2416792计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
GVB° is a basic buffer that can be used to make other traditional buffers used for complement assays. Ca⁺⁺ and Mg⁺⁺ may be added to GVB° to make GVB⁺⁺ for classical pathway and lectin pathway assays. MgEGTA may be added to GVB° for alternative pathway assays. EDTA may be added to GVB° to prepare GVBE to inhibit complement activation. GVB° is also used for dilution of serum and other assay components in many complement assays especially in alternative pathway assays (Morgan, B.P. (2000; Dodds, A.W. and Sim, R.B. (1997)).
Buffer Components
Veronal is used as the buffer because in the mid-1900s this was the only buffer for pH range 7.2-7.4 that did not chelate metal ions and did not to inhibit complement reactions as did other buffers. Sodium chloride is present to provide an isotonic environment so that cells do not lyse due to osmotic pressure. Gelatin is present to prevent loss of protein components due to adsorption onto tips or tubes during dilutions and in the assays themselves. Azide is present to prevent bacterial growth.
Physical Characteristics
The concentration of gelatin in this buffer is below the concentration that forms solid gels. However, because of the gelatin is close to its gelling concentration at 4℃ some strings of gelatin form during standing at this temperature. They can be redissolved easily by heating to 37℃or by brief heating in a microwave oven.
Applications
GVB° should be used to prepare Er and to wash them before their use in assays. GVB° is used to dilute all of the components of alternative pathway assays. The General Description section above describes the use of GVB° to prepare GVB⁺⁺ , MgEGTA for alternative pathway assays and EDTA to prepare GVBE. GVB° is also used for dilution of serum and other assay components in many complement assays especially in alternative pathway assays.
Buffer Components
Veronal is used as the buffer because in the mid-1900s this was the only buffer for pH range 7.2-7.4 that did not chelate metal ions and did not to inhibit complement reactions as did other buffers. Sodium chloride is present to provide an isotonic environment so that cells do not lyse due to osmotic pressure. Gelatin is present to prevent loss of protein components due to adsorption onto tips or tubes during dilutions and in the assays themselves. Azide is present to prevent bacterial growth.
Physical Characteristics
The concentration of gelatin in this buffer is below the concentration that forms solid gels. However, because of the gelatin is close to its gelling concentration at 4℃ some strings of gelatin form during standing at this temperature. They can be redissolved easily by heating to 37℃or by brief heating in a microwave oven.
Applications
GVB° should be used to prepare Er and to wash them before their use in assays. GVB° is used to dilute all of the components of alternative pathway assays. The General Description section above describes the use of GVB° to prepare GVB⁺⁺ , MgEGTA for alternative pathway assays and EDTA to prepare GVBE. GVB° is also used for dilution of serum and other assay components in many complement assays especially in alternative pathway assays.
规格
产品名称
GVB°(GVB without Ca⁺⁺ and Mg⁺⁺)
别名
GVB°(不含Ca⁺⁺和Mg⁺⁺的GVB)
规格或纯度
0.1 % gelatin, 5 mM Veronal, 145 mM NaCl, 0.025 % NaN3 , pH 7.3.
分子类型
小分子
储存与运输
储存缓冲液
Sodium veronal
浓度
0.1 % gelatin, 5 mM Veronal, 145 mM NaCl, 0.025 % NaN3 , pH 7.3.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
技术文档
常见问题
本产品应如何储存？
请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）