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睾丸假单胞菌中的羟基类固醇脱氢酶
有货
为什么选择此级别
EnzymoPure™ 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Hydroxysteroid dehydrogenases catalyze the interconversion of hydroxyl and carboxyl groups of steroids. P.testosteroni derived hydroxysteroid dehydrogenases are of two types: 3-α-hydroxysteroid dehydrogenase (α enzyme) and 3-β-hydroxysteroid dehydrogenase (β enzyme). The α enzyme has a molecular weight of 47,000 daltons. The α enzyme oxidizes only 3-α-hydroxysteroids of the C19, C21 and C24 series. It is inhibited by heavy metals and sulfhydryl-binding agents. The β enzyme catalyzes the oxidation of 3-β-hydroxysteroids of the C19 and C21 series, 17-β-hydroxysteroids of the C18, C19 and C21 series, as well as certain 16-β-hydroxysteroids. It is inhibited by heavy metals and reducing agents. The oxidation of testosterone is inhibited by 3,17-α-estradiol and other 1,3,5-estradiene derivatives. Aladdin supplies two preparations: one from the regular P. testosteroni (ATCC 11966) culture which produces both the α and the β enzymes, and a second from a mutant strain which produces almost exclusively the α enzyme. By using both, the β-hydroxysteroid can be determined by difference.
A purified powder obtained from adapted cells of a mutant strain. Activity on androsterone only, no activity exhibited on testosterone.H128454 contains only α activity.
规格
产品名称
睾丸假单胞菌中的羟基类固醇脱氢酶
规格或纯度
EnzymoPure™, ≥0.5 units/mg dry weight
分子类型
酶
储存与运输
浓度
≥0.5 units/mg dry weight
储存条件
-20°C储存
运输条件
超低温运输
单位定义
One Unit reduces one micromole of NAD per minute at 25°C, pH 9.0 using androsterone or testosterone as substrate.
技术文档
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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