BioReagent,无菌过滤 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
储存缓冲液：0.05% ProClin 300
是否无菌：是
Non-specific monocyte binding remains a ubiquitous challenge in flow cytometry. Although the exact mechanism is undefined, literature implicates CD64 interactions with cyanine dyes. However, our data demonstrate that non-specific binding extends beyond cyanine-based fluorophores: certain non-cyanine fluorescent dyes also exhibit this phenomenon. Crucially, we observed selective non-specific staining on monocytes for all tested PE and APC tandem dyes. Human Fc Receptor Blocking Solution is a non-antibody blocking solution and has been formulated to block non-specific binding of PE-Cy7, PE-Cy5, PE-Cy5.5, PE-APC, APC-Cy7, and AF647 which is also a cyanine dye commonly appeared on monocytes and macrophages.
This buffer contains specialized human IgG containing 0.05% ProClin 300. It is not recommended to be used for staining human IgG. Handle as biohazard agent.
Human peripheral blood leukocytes were either not preincubated (Upper Plots) or were preincubated (Lower Plots) with Human Fc Receptor Blocking Solution as indicated. The cells were then selectively stained with either Mouse IgG1-kappa (PE-Cy5.5), anti-E8L (Ab216584), Mouse IgG1-kappa (PE-Cy7), anti-E8L (Ab216583), Mouse IgG2b-kappa (PE-Cy5), anti-E8L (Ab216565), Mouse IgG2c-kappa (PE-Cy5), anti-HEL (Ab212231) or Mouse IgG2c-kappa (PE-Cy7), anti-HEL (Ab212232). Two-dimensional pseudocolor plots of SSC-A vs Mouse IgG show that the Human Fc Receptor Blocking Solution reduces non-specific binding compared to staining without any blocking reagent.
Human peripheral blood leukocytes were either not preincubated any blocking reagent (Top Plots) or were preincubated with competitor Fc Receptor Blocking reagent (Middle Plots) or were preincubated with Human Fc Receptor Blocking Solution (Bottom Plots) as indicated. The cells were then selectively stained with either Mouse IgG (APC-Cy7) (Ab213860), Mouse IgG2b-kappa (PE-Cy5), anti-E8L (Ab216565) or Mouse IgG2c-kappa (PE-Cy5), anti-HEL (Ab212231). Two-dimensional pseudocolor plots of SSC-A vs Mouse IgG show that the Human Fc Receptor Blocking Solution reduces non-specific binding compared to staining without any blocking reagent or with competitor Fc Receptor Blocking reagent.
Human Fc Receptor Blocking Solution (H1374078) - Flow Cytometry
Human peripheral blood mononuclear cells were either untreated (black) or treated with Human Fc Receptor Blocking Solution (H1374078) (red) and stained with recombinant CD14 Antibody (AF405) (Ab214465) and CD3 Mouse mAb (PE-Cy5) (Ab189665) (left), CD3 Mouse mAb (PE-Cy7) (Ab189666) (middle), or CD3 Mouse mAb (AF647) (Ab189663).
Human Fc Receptor Blocking Solution (H1374078) - Flow Cytometry
Flow Cytometry analysis of no Human Fc Receptor Blocking Solution (left) or with Human Fc Receptor Blocking Solution (H1374078) (right) blocked human peripheral blood monocyte labelling CD11b (red) with CD11b Mouse mAb (PE-Cy5) (Ab191617) at 1.1 μg/mL for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy5) (Ab190078). Black - Unlabelled control, cells without incubation with primary antibody.
Human Fc Receptor Blocking Solution (H1374078) - Flow Cytometry
Human peripheral blood leukocytes were either not preincubated (Left Plots) or were preincubated (Right Plots) with Human Fc Receptor Blocking Solution (H1374078) as indicated. The cells were then stained with Mouse IgG (PE-Cy5) (Ab190078). Two-dimensional pseudocolor plots of SSC-A vs Mouse IgG show that the Human Fc Receptor Blocking Solution reduces non-specific binding compared to staining without any blocking reagent.
10 μL/test.
It is recommended to add 10 µL of Human Fc Receptor Blocking Solution per million cells in 100 µl staining volume, mix and incubate at 4 °C for 30 minutes prior to staining with antibody of inter
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0825781 |
分析证书 | H1374078 |
| ZJ25F0825782 |
分析证书 | H1374078 |
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