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HUP-55 is a prolyl endopeptidase inhibitor ( IC 50 = 5 nM). HUP-55 reduces the dimerization of α-synuclein in Neuro2a cells and induces autophagy ( Autophagy ) in HEK293 cells. It also decreases the increase in reactive oxygen species ( ROS ) production induced by hydrogen peroxide in SH-SY5Y cells at a concentration of 10 µM. In a mouse Parkinson’s disease model, HUP-55 (10 mg/kg) improves motor function (reduces the use frequency of the impaired paw) and decreases the levels of harmful oligomers of α-synuclein in the striatum caused by overexpression of α-synuclein.