20-Hydroxylucidenic acid E2 is a triterpenoid compound found in Ganoderma lucidum. It exhibits significant inhibitory effects on inflammation induced by TPA. Additionally, 20-Hydroxylucidenic acid E2 can suppress the expression of EBV early antigen induced by TPA , with a IC 50 of 290 mol ratio/32 pmol TPA (meaning that when the molar concentration of this compound is 290 times that of 32 pmol of TPA, it can inhibit 50% of EBV early antigen expression). Therefore, 20-Hydroxylucidenic acid E2 has potential applications in anti-inflammatory and anticancer research.