5-HT6 inverse agonist 1 (Compound 33) is an antagonist for 5-HT6 receptor with a K i of 23 nM and a K b of 6.62 nM. 5-HT6 inverse agonist 1 inhibits the 5-HT6R mediated Cdk5 and mTOR signaling pathway. 5-HT6 inverse agonist 1 reduces tactile hypersensitivity in spinal nerve ligation (SNL)-induced rat model.