hAChE-IN-3 (compounds 5c) is a potent and blood-brain barrier permeable AChE , BuChE , MAO-B-IN-1 and BACE-1 inhibitor, with IC 50 values of 0.44, 0.08, 5.15 and 0.38 μM, respectively. hAChE-IN-3 has antioxidant activity and metal chelating ability. In addition, hAChE-IN-3 can bind to peripheral anion sites, and affect β amyloid and reduce Alzheimer's-associated neurodegeneration. hAChE-IN-3 has the potential for the research of Alzheimer's disease.