hAChE-IN-7 (compound 5s) is a mixed inhibitor affecting both the catalytic active site (CAS) and peripheral anionic site (PAS) of hAChE. hAChE-IN-7 displays the balanced inhibitory effect on hAChE ( IC 50 =69.8 nM) and hBuChE ( IC 50 =68.0 nM), and exhibits inhibitory activity against β-secretase-1 (BACE-1) (IC 50 =3.6 μM). hAChE-IN-7 has the potential for Alzheimer's disease (AD) research.