计算溶液所需的质量、体积或浓度。
BioReagent,无菌,蛋白组学级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
复溶:0.1% Formic acid.
HeLa蛋白消化标准品是一种用于质谱分析的蛋白质样品,源自HeLa细胞,经酶解处理。其主要作用包括质量控制、方法开发、定量分析、蛋白质鉴定、修饰分析等,是质谱分析中不可或缺的工具,可提高分析的准确性和可靠性。
储存与运输:
储存:冻干粉状态在-20°C或更低温条件下稳定保存2年,复溶分装产品在-80°C可保存1年,避免反复冻融!
运输:建议冰袋运输,保持低温。
操作流程:
1、准备:推荐使用0.1%FA (甲酸)溶液溶解产品。如:取1管20μg的HeLa蛋白消化标准品冻干粉,加入40μL 0.1%FA (甲酸) 溶液溶解,可进行Votex震荡溶解,终浓度为0.5μg/μL,分装保存。
2、上样:使用适当的量进行上样,一般60min梯度建议500ng,120min梯度建议1-2μg,将肽段标准品溶液注入质谱仪进行分析。
注意事项和免责说明:
1、本品仅限于专业人员的科学研究使用,不得用于临床诊断或治疗,不得用于食品或药品。
2、为了您的安全和健康,请穿实验服并佩戴-次性手套和口罩操作。
The HeLa protein digestion standard is a protein sample derived from HeLa cells and subjected to enzymatic hydrolysis, which is used for mass spectrometry analysis. Its main functions include quality control, method development, quantitative analysis, protein identification, and modification analysis. It is an indispensable tool in mass spectrometry analysis, capable of improving the accuracy and reliability of the analysis.
Storage and Transportation:
Storage: The lyophilized powder is stable for 2 years when stored at -20°C or below. The reconstituted and aliquoted product can be stored at -80°C for 1 year. Avoid repeated freezing and thawing!
Transportation: It is recommended to transport with ice packs to maintain low temperature.
Operating Procedures:
1.Preparation: It is recommended to dissolve the product with 0.1% FA (formic acid) solution. For example: take 1 tube of 20μg HeLa protein digestion standard lyophilized powder, add 40μL of 0.1% FA (formic acid) solution to dissolve it, and vortex to dissolve. The final concentration is 0.5μg/μL, and aliquot for storage.
2.Loading: Use an appropriate amount for loading. Generally, 500ng is recommended for a 60-minute gradient, and 1-2μg for a 120-minute gradient. Inject the peptide standard solution into the mass spectrometer for analysis.
Precautions and Disclaimer:
1.This product is limited to scientific research use by professionals, and shall not be used for clinical diagnosis or treatment, nor for food or drugs.
2.For your safety and health, please wear a lab coat and disposable gloves and masks during operation.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | H1456336 | |
| 分析证书 | H1456336 | |
| 分析证书 | H1456336 | |
| 分析证书 | H1456336 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Proteomics grade 级别指南 → 查看 Sterile 级别指南 →