BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒采用双抗体夹心ELISA法检测样品中人脑源性神经营养因子的浓度。人脑源性神经营养因子捕获抗体已经预包被于酶标板上，当加入样品或标准品时，其中的人脑源性神经营养因子会与捕获抗体结合，而其它游离成分则会通过洗涤被除去。接着，再加入生物素标记的人脑源性神经营养因子抗体后，抗人脑源性神经营养因子抗体与人脑源性神经营养因子接合，形成夹心的免疫复合物，其它游离成分则通过洗涤被除去。随后加入酶复合物，生物素与酶复合物特异性结合，这样酶复合物上的HRP就与夹心的免疫复合物连接起来，而其它游离成分则通过洗涤被除去。最后加入显色剂，若样品中存在人脑源性神经营养因子，则会形成免疫复合物，其上连接的HRP会催化无色的显色剂氧化生成蓝色物质，而后加入终止液，最终产物呈黄色。通过酶标仪检测，读取450nm处的OD值，人脑源性神经营养因子浓度与OD450值之间呈正比，通过检测标准品绘制标准曲线，对照未知样品中OD值，即可计算出样品中人脑源性神经营养因子的浓度。
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human BDNF concentration in samples. The Human BDNF capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human BDNF present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human BDNF is added. This antibody binds to the captured Human BDNF, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human BDNF is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman BDNF is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofHuman BDNF, and the concentration of Human BDNF in unknown samples is interpolated from this curve based on their OD values.
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