计算溶液所需的质量、体积或浓度。
人源树突状细胞因子套装
有货
为什么选择此级别
BioReagent,无菌过滤 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
人类树突状细胞细胞因子包中含有 IL-4 和 GM-CSF，这是培养人类树突状细胞所必需的关键成分。重组人 IL-4 是一种在体外促进人类树突状细胞分化和成熟所必需的细胞因子。IL-4 蛋白，特别是重组 IL-4，能够促使从单核细胞等前体细胞中发育出树突状细胞。人类 IL-4 与其他因素（包括 GM-CSF（粒细胞-巨噬细胞集落刺激因子））协同作用，推动分化过程。通常情况下，将单核细胞在含有重组 IL-4 和 GM-CSF 的环境中培养，可得到未成熟的树突状细胞，这些细胞随后可通过其他刺激进一步成熟。
该包装内含以下2个小瓶：
重组人IL-4蛋白（货号：rp156008）
无动物源重组人GM-CSF蛋白（货号：rp186551）
白细胞介素 - 4 是一种多效性细胞因子，可调节多种 T 细胞和 B 细胞反应，包括细胞增殖、存活和基因表达。由肥大细胞、T 细胞和骨髓基质细胞产生，白细胞介素 - 4 调节初始 CD4+ T 细胞向辅助性 Th2 细胞的分化。
GM-CSF 是一种造血生长因子，它能刺激中性粒细胞和巨噬细胞的发育，并促进早期红系巨核细胞和嗜酸性祖细胞的增殖与发育。
信息
| 名称 | 种属 | 表达系统 | 标签 | 纯度 | 活性 | 内毒素 | 外观 | 储存缓冲液 | 复溶 |
| 重组人IL-4蛋白 | 人 | E. coli | N-His | ≥95% (SDS-PAGE) | Recombinant Human IL‑4 Protein (rp156008) stimulates cell proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.31 ng/mL. | <1.0 EU/μg | 冻干(Lyophilized) | Lyophilized from 10mM PBS, pH 7.4 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 无动物源重组人GM-CSF蛋白 | 人 | 293F | No tag | ≥95% (SDS-PAGE) | Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 90.7 pg/mL. | <0.1 EU/μg | 冻干(Lyophilized) | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4. | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
注意事项：
1、本产品仅作科研用途。
2、为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
The Human Dendritic Cell Cytokine Package contains IL-4 and GM-CSF, key components required for the culturing of murine dendritic cells. Recombinant murine IL-4 is an essential cytokine used in the differentiation and maturation of murine dendritic cell in vitro. The IL-4 protein, specifically recombinant IL-4, promotes the development of dendritic cells from precursor cells such as monocytes. Murine IL-4 acts synergistically with other factors, including GM-CSF (granulocyte-macrophage colony stimulating factor), to drive the differentiation process. Typically, monocytes are cultured in the presence of recombinant IL-4 and GM-CSF to yield immature dendritic cells, which can then be futher matured by additional stimuli.
The package contains the following two vials:
Recombinant Human IL-4 Protein（Cat. No. rp156008）
Animal-Free Recombinant Human GM-CSF Protein （Cat. No. rp186551）
IL-4 is a pleiotropic cytokine that regulates diverse T- and B-cell responses including cell proliferation, survival, and gene expression. Produced by mast cells, T cells, and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells.
Information
| Name | Species | Expression System | Tag | Purity | Activity | Endotoxin | Appearance | Storage | Reconstitution |
| Recombinant Human IL-4 Protein | Human | E. coli | N-His | ≥95% (SDS-PAGE) | Recombinant Human IL‑4 Protein (rp156008) stimulates cell proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.31 ng/mL. | <1.0 EU/μg | Lyophilized | Lyophilized from 10mM PBS, pH 7.4 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Animal-Free Recombinant Human GM-CSF Protein | Human | 293F | No tag | ≥95% (SDS-PAGE) | Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 90.7 pg/mL. | <0.1 EU/μg | Lyophilized | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4. | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Notes:
1. This product is for research use only.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
规格或纯度
无菌过滤, BioReagent
英文名称
Human Dendritic Cell Cytokine Package
应用
Biochemical assay, Cell Culture, Functional Assay, Functional Studies
储存与运输
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20~-80℃ 存储12个月；复溶后建议分装。避免反复冻融。
组分与储存
| A1511079 | Components | Appearance | Size 1 | Size 2 | Size 3 | Storage | Quantity Per Test |
| A1511079A | 重组人IL-4蛋白 | Lyophilized | 10 μg | 50 μg | 100 μg | -20°C | Use as per requirements |
| A1511079B | 无动物源重组人GM-CSF蛋白 | Lyophilized | 10 μg | 50 μg | 100 μg | -20°C | Use as per requirements |
技术文档
高级数据
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产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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