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肝脂酶(hepaticlipase,HL)是一种脂肪分解酶,在肝实质细胞中合成,存在于肝脏窦周间隙内皮细胞表面和窦周间隙腔面的肝细胞微绒毛表面,可水解各种脂蛋白中的甘油三酯(TG)和磷脂(PL),使各种脂蛋白颗粒的大小和密度发生变化,当血浆中的 HL 及其活性增高时,可导致血浆中低密度脂蛋白(LDL)水平升高,加速动脉粥样硬化的发生与发展。HL 水解α-乙酸萘酯产生α-萘酚,可与固蓝 B 盐形成紫红色偶氮化合物,在 595 nm 有特征吸收峰,其颜色深浅在一定范围内与HL 活性成正相关。
Hepatic lipase (HL) is a lipolytic enzyme synthesized in hepatic parenchymal cells and localized on the surface of endothelial cells lining the hepatic sinusoids and on the microvilli of hepatocytes facing the space of Disse. It hydrolyzes triglycerides (TG) and phospholipids (PL) in various lipoproteins, altering the size and density of lipoprotein particles. Elevated HL levels and activity in plasma can lead to increased levels of low-density lipoprotein (LDL) in the blood, accelerating the occurrence and development of atherosclerosis. HL hydrolyzes α-naphthyl acetate to produce α-naphthol, which forms a purple-red azo compound with Fast Blue B salt. This compound has a characteristic absorption peak at 595 nm, and within a certain range, the color intensity is positively correlated with HL activity.