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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
β-羟丁酸在检测糖尿病酮症酸中毒诊断、治疗中有重要意义，对糖尿病的早期诊断也有重要意义。β-羟丁酸在β-羟丁酸脱氢酶催化下生成乙酰乙酸。同时氧化型辅酶Ⅰ被还原成还原型辅酶Ⅰ即NADH，NADH 接着与 WST-8 显色剂生成于 450nm 有特征吸收峰的黄色物质，通过检测该黄色物质于 450nm 处的增加量，即可计算得出样本中β-羟丁酸含量。
β-Hydroxybutyrate plays an important role in the diagnosis and treatment of diabetic ketoacidosis and is also of great significance for the early diagnosis of diabetes. Under the catalysis of β-hydroxybutyrate dehydrogenase, β-hydroxybutyrate is converted into acetoacetate. At the same time, oxidized coenzyme I (NAD⁺) is reduced to reduced coenzyme I (NADH). NADH then reacts with the WST‑8 chromogenic agent to form a yellow product with a characteristic absorption peak at 450 nm. The β-hydroxybutyrate content in the sample can be calculated by measuring the increase in this yellow product at 450 nm.
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