计算溶液所需的质量、体积或浓度。
BioReagent,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
LB 是分子生物学最广泛使用的细菌培养基,可用于大肠杆菌、恶臭假单胞菌、副溶血性弧菌、芽孢杆菌等细菌的培养,配制简单,营养丰富,含有多肽、氨基酸、水溶性维生素和碳水化合物,可用于基因克隆、质粒DNA 生产和重组蛋白生产等实验。 高密度 LB 是采用精炼蛋白胨和酵母粉开发的专门用于大肠杆菌培养的 LB 培养基,比普通 LB 含有更丰富的氮源、多肽和氨基酸,用于 DH5a 等克隆菌株培养时,菌体产量和质粒产量比普通 LB 高出约 50%;用于 BL21 DE3 等原核表达菌株培养时菌体产量比普通 LB 高出 20%-50%。
产品组分:
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使用方法:
高密度 LB with Agar 粉剂培养基:取一袋 (20g),加双蒸水 450ml,待 Tryptone,NaCl,Yeast Extract溶解后 (Agar 不溶解),定容到 0.5L,121℃ 高压灭菌 20min,灭菌后温度降到 55 ℃以下使用,或放室温保存。
注意事项:
1、pH 值 7.0±0.1( 25℃),本产品加入 pH=7.0 的去离子水后 pH 接近 7.0,可不调 pH 值直接使用。
2、LB 培养基需先补水,121℃-20min 高压灭菌后使用。
3、若发现有严重吸潮现象,停止使用。
4、高密度 LB 培养基培养大肠杆菌时生长速度更快,比普通 LB 培养基提高约 20%。
5、若配制少于 0.5L,按每 100ml 加 2.5g 培养基的比例加入即可,剩余培养基封口后放干燥处保存。
LB medium is the most widely used bacterial culture medium in molecular biology. It supports the cultivation of Escherichia coli, Pseudomonas putida, Vibrio parahaemolyticus, Bacillus species and other bacteria. Easy to prepare and nutrient-rich, it contains peptides, amino acids, water-soluble vitamins and carbohydrates, and is applicable to experiments including gene cloning, plasmid DNA production and recombinant protein expression.High Density LB is a special LB medium developed with refined tryptone and yeast extract for culturing E. coli. It provides more abundant nitrogen sources, peptides and amino acids than standard LB medium. When culturing cloning strains such as DH5α, it yields approximately 50% more cell mass and plasmid DNA than regular LB. For prokaryotic expression strains like BL21 (DE3), its biomass output is 20%–50% higher than conventional LB.
Product Composition:
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Instructions for Use:
High-Density LB Agar (Premixed Powder): Weigh one pouch (20g) and add into 450 mL double-distilled water. Wait until tryptone, NaCl and yeast extract are fully dissolved (agar remains undissolved), then adjust the final volume to 0.5 L. Autoclave at 121°C for 20 minutes. Cool the medium to below 55°C before use, or store the sterilized medium at room temperature.
Precautions:
1. pH value (25°C): 7.0±0.1. When this product is mixed with pH 7.0 deionized water, the pH of the medium will be close to 7.0, so it can be used directly without pH adjustment.
2. LB medium shall be prepared with water first and autoclaved at 121°C for 20 minutes before use.
3. Discontinue use if severe moisture absorption of the powder is observed.
4. High Density LB medium supports faster growth of Escherichia coli, with a growth rate about 20% higher than standard LB medium.
5. For preparation volumes less than 0.5 L, add 2.5g medium powder per 100 mL water. Seal the leftover powder tightly and store it in a dry place.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | H1524572 |